Anti-PLA2R antibody [EPR20483]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (AB211573)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PLA2R with ab211573 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Negative control : No staining on human pancreas (PMID : 7721806).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [EPR20483] (AB211573)

Immunohistochemical analysis of paraffin-embedded human kidney tissue (from a patient with renal clear cell carcinoma) labeling PLA2R with ab211573 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Positive staining on glomerular basement membrane of human kidney (PMID : 26222864).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-PLA2R antibody [EPR20483] (AB211573)

Blocking/Dilution buffer : 5% NFDM/TBST.

PLA2R is specifically expressed in kidney (PMID : 7721806).

All lanes:

Western blot - Anti-PLA2R antibody [EPR20483] (ab211573) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 50 µg

Lane 2:

Human fetal liver lysate at 50 µg

Lane 3:

Human fetal heart lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 169 kDa

Observed band size: 180 kDa

false

Exposure time: 3min

• WB

CiteAb

Western blot - Anti-PLA2R antibody [EPR20483] (AB211573)

PLA2R western blot using anti-PLA2R antibody [EPR20483] ab211573. Publication image and figure legend from Friedemann, M., Gutewort, K., et al., 2020, Sci Rep, PubMed 32493972.

ab211573 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab211573 please see the product overview.

Cell proliferative behaviour in PLA2R1-transfected Jurkat cells (Jurkat-PLA2R1) compared to control vector-transfected Jurkat cells (Jurkat-Ctrl). (A) The amount of PLA2R1-mRNA was determined using RT-qPCR with β-actin as reference gene. Results are shown as mean ± SD of three independent experiments (biological n = 3) with two technical replicates. Bar graphs represent the ratio of PLA2R1 and β-actin gene expression. Symbol # indicates that PLA2R1 expression in Jurkat-Ctrl cells was not detected after 45 PCR cycles and therefore set to zero. (B) The level of PLA2R1 protein expression was assessed using western blot with actin as reference protein and human recombinant PLA2R1 as positive control. A cropped representative blot section (from four independent experiments) is shown (biological n = 4, full-length blot is presented in Supplementary Fig. 6). (C–E) Results are the means ± SD of three independent experiments (biological n = 3) with two technical replicates. 2 × 105 Jurkat cells were seeded in 6-well plates and trypan blue-negative (C) and -positive Jurkat cells (D) were counted during four consecutive days. (E) Apoptosis was stimulated with hydrogen peroxide for 24 h and determined by Annexin-V-Fluorescein/Hoechst 33258 staining using flow cytometry analysis. Levels of significance are defined as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).

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