AB33

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]

Name: Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) | Abcam
Brand: Abcam Limited
SKU: AB33
Price: 390 USD
Availability: InStock

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(6 Publications)

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) is a mouse monoclonal antibody detecting Placental alkaline phosphatase (PLAP) in Western Blot. Suitable for Human.

- Trusted since 1998

View Alternative Names

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

1 Images

Lab

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (AB33)

Blocking buffer : 3% milk.

Gel Type : MOPS.

All lanes:

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) at 5 µg/mL

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

Human kidney tissue lysate (negative control) at 10 µg

Lane 3:

Human liver tissue lysate (negative control) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 65 kDa

false

Exposure time: 8min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B6

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Placental alkaline phosphatase (PLAP)?
Anti-Placental alkaline phosphatase (PLAP) [8B6] (ab33) specifically detects a band for Placental alkaline phosphatase (PLAP) (UniProt: P05187) at a molecular weight of 58kDa.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 38:1381-1391 PubMed29650693

2018

High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Yae Hu,Ruhong Yan,Ce Zhang,Zhichao Zhou,Meng Liu,Can Wang,Hong Zhang,Liang Dong,Tiantian Zhou,Yi Wu,Ningzheng Dong,Qingyu Wu

Journal of cell science 130:2747-2756 PubMed28687622

2017

Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP.

Applications

Unspecified application

Species

Unspecified reactive species

Werner E G Müller,Shunfeng Wang,Meik Neufurth,Maria Kokkinopoulou,Qingling Feng,Heinz C Schröder,Xiaohong Wang

PloS one 5:e13130 PubMed20976190

2010

Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.

Applications

ICC/IF

Species

Human

Tyler M Sharp,Susana Guix,Kazuhiko Katayama,Sue E Crawford,Mary K Estes

The journal of gene medicine 7:354-65 PubMed15619290

2004

Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo.

Applications

ELISA

Species

Human

Lei Zhang,Qixin Leng,A James Mixson

International journal of cancer. Supplement = Journal international du cancer. Supplement 2:59-66 PubMed3162446

1988

Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.

Applications

Unspecified application

Species

Unspecified reactive species

H Durbin,E M Milligan,S J Mather,D F Tucker,R Raymond,W F Bodmer

International journal of cancer. Supplement = Journal international du cancer. Supplement 2:50-8 PubMed2450848

1988

Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.

Applications

Unspecified application

Species

Unspecified reactive species

H Durbin,D F Tucker,E M Milligan,L G Bobrow,P H Warne,Y L Pookim,W F Bodmer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (AB33)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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