AB133602

Anti-ALPP+ALPG+ALPI antibody [EPR6141]

Name: Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (ab133602) | Abcam
Brand: Abcam Limited
SKU: AB133602
Price: 405 USD
Availability: InStock
Rating: 4 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(29 Publications)

Rabbit Recombinant Monoclonal PPB1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 29 publications.

View Alternative Names

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Lanes 1-4 : Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (ab133602) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ALPP knockout HeLa cell lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Predicted band size: 58 kDa

Observed band size: 70 kDa

false

Unknown

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

All lanes:

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (ab133602) at 1/10000 dilution

Lane 1:

Human placenta tissue lysate

Lane 2:

Human seminoma tissue lysate

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 70 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing positive staining in Seminoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-ALPP+ALPG+ALPI antibody [EPR6141] - BSA and Azide free
HRP

HRP Anti-ALPP+ALPG+ALPI antibody [EPR6141]
578 PE

PE Anti-ALPP+ALPG+ALPI antibody [EPR6141]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ALPP+ALPG+ALPI antibody [EPR6141]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ALPP+ALPG+ALPI antibody [EPR6141]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ALPP+ALPG+ALPI antibody [EPR6141]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ALPP+ALPG+ALPI antibody [EPR6141]
660 APC

APC Anti-ALPP+ALPG+ALPI antibody [EPR6141]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-ALPP+ALPG+ALPI antibody [EPR6141]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6141

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

Additional targets

ALPG,ALPI

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 50:144-157 PubMed40171108

2025

Moderate static magnetic field regulates iron metabolism and salvage bone loss caused by iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxiao Zhen,Shenghang Wang,Jiancheng Yang,Gejing Zhang,Chao Cai,Jianping Wang,Aifei Wang,Youjia Xu,Yanwen Fang,Min Wei,Dachuan Yin,Xinle Luo,Ming Gong,Hao Zhang,Peng Shang

Frontiers in cell and developmental biology 13:1528714 PubMed39996028

2025

The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions.

Applications

Unspecified application

Species

Unspecified reactive species

Kunfeng Bai,Xintong Li,Yanjie Guo,Ye Shang,Leqian Lin,Philip C N Chiu,Cheuk-Lun Lee

Placenta 160:118-125 PubMed39793469

2025

Placental alkaline phosphatase (PLAP): Is it exclusively placental?

Applications

Unspecified application

Species

Unspecified reactive species

Awanit Kumar,Sourabh Sharma,Maged M Costantine,Kara Rood,Rheanna Urrabaz-Garza,Jeena Jacob,Lauren S Richardson,Ananth Kumar Kammala,Ramkumar Menon

Journal of extracellular biology 3:e135 PubMed38938672

2024

Differential response of placental cells to high D-glucose and its impact on extracellular vesicle biogenesis and trafficking via small GTPase Ras-related protein RAB-7A.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Palma,Andrew Lai,Katherin Scholz-Romero,Haarika Chittoory,Benjamin Van Haeringen,Flavio Carrion,Aase Handberg,Martha Lappas,Sunil R Lakhani,Amy E McCart Reed,H David McIntyre,Soumyalekshmi Nair,Carlos Salomon

Advanced healthcare materials 13:e2302879 PubMed37927129

2023

3D Printed Photothermal Scaffold Sandwiching Bacteria Inside and Outside Improves The Infected Microenvironment and Repairs Bone Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Youzi Zhao,Honglei Kang,Yuhao Xia,Lingshun Sun,Feng Li,Honglian Dai

Journal of translational medicine 21:496 PubMed37488572

2023

Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Jiexing He,Haihua Liu,Qiuyu Huang,Shuoshi Wang,Ailan Yin,Shuying Chen,Xinyang Shen,Yanxuan Xiao,Haoyue Hu,Jiayi Jiang,Wenqian Chen,Song Wang,Zhenqin Huang,Jiaqi Li,You Peng,Xiaocong Wang,Xinping Yang,Zhijian Wang,Mei Zhong

International journal of nanomedicine 18:641-657 PubMed36789391

2023

Gestational Diabetes Mellitus Impedes Fetal Lung Development Through Exosome-Dependent Crosstalk Between Trophoblasts and Lung Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengzheng Chen,Mengqi Gu,Shuting Wan,Xiaotong Jiang,Fengyuan Zhang,Yuchen Li,Qian Zhou,Yuan Lu,Lei Li,Xietong Wang

Open life sciences 17:1591-1599 PubMed36518887

2022

PHF8 promotes osteogenic differentiation of BMSCs in old rat with osteoporosis by regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Kai Huang,Hongbin Dai,Chunhe Sha

Cells 11: PubMed36359855

2022

Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Zhang,Qi Wu,Shuqi Zhu,Yibo Tang,Yanmin Chen,Danqing Chen,Zhaoxia Liang

Cell reports 39:110962 PubMed35705034

2022

Genetic encoding of an esophageal motor circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana C Coverdell,Ruei-Jen Abraham-Fan,Chen Wu,Stephen B G Abbott,John N Campbell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALPP+ALPG+ALPI antibody [EPR6141]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (29)

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