Rabbit Recombinant Monoclonal PPB1 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695), expandable thumbnail](https://content.abcam.com/products/images/placental-alkaline-phosphatase-plap-antibody-sp15-ab16695--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117872.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)")
![Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695), expandable thumbnail](https://content.abcam.com/products/images/placental-alkaline-phosphatase-plap-antibody-sp15-ab16695--flow-cytometry-intracellular-img414044.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)")
Publications
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- Stem cell research & therapy 12:642021The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Zhou,Siyu Liu,Wei Wang,Qiang Sun,Mengzhu Lv,Shude Yang,Shuang Tong,Shu GuoPubMed 33461605
- The Journal of biological chemistry 295:16393-164102020Calnexin mediates the maturation of GPI-anchors through ER retention.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Yu Guo,Yi-Shi Liu,Xiao-Dong Gao,Taroh Kinoshita,Morihisa FujitaPubMed 32967966
- Genesis (New York, N.Y. : 2000) 58:e233592020Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoire Cardot-Ruffino et. al.PubMed 32191380
- Cells, tissues, organs 207:97-1132019Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel N Bracey et. al.PubMed 31655811
- eLife 8:2019Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Ashley et. al.PubMed 30714906
- Retrovirology 10:42013Virion stiffness regulates immature HIV-1 entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Bo Pang et. al.PubMed 23305456
- The Journal of biological chemistry 287:32525-342012Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.
- Journal of andrology 33:505-132011Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice.Applications:IHC-PReactive species:MouseCamelia M Saffarini,Nicholas E Heger,Hideki Yamasaki,Tao Liu,Susan J Hall,Kim BoekelheidePubMed 21868749
- Gynecologic oncology 122:560-62011Comparison of candidate serologic markers for type I and type II ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Lu et. al.PubMed 21704359
- Endocrinology 151:929-38201017beta-estradiol accelerates tumor onset and decreases survival in a transgenic mouse model of ovarian cancer.Applications:IHC-PReactive species:MouseLaura A Laviolette,Kenneth Garson,Elizabeth A Macdonald,Mary K Senterman,Kerri Courville,Colleen A Crane,Barbara C VanderhydenPubMed 20056833
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Alternative names
PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Recommended products
Rabbit Recombinant Monoclonal PPB1 antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP15
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
- Epitope
- Not determined
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was switched from a hybridoma to recombinant production method on 7th March 2019.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.
- Biological function summary
PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.
- Pathways
PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.
- Associated diseases and disorders
PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695), expandable thumbnail](https://content.abcam.com/products/images/placental-alkaline-phosphatase-plap-antibody-sp15-ab16695--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117872.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
Human seminoma stained with ab16695.
This image was generated from the hybridoma version.
![Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695), expandable thumbnail](https://content.abcam.com/products/images/placental-alkaline-phosphatase-plap-antibody-sp15-ab16695--flow-cytometry-intracellular-img414044.jpg "Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)")
Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.
This image was generated from the hybridoma version.
Downloads
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com