AB16695

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(13 Publications)

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695) is a rabbit monoclonal antibody detecting Placental alkaline phosphatase (PLAP) in Flow Cytometry (Intra), IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2005

View Alternative Names

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

2 Images

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

This image was generated from the hybridoma version.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Human seminoma stained with ab16695.

This image was generated from the hybridoma version.

Carrier free

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP15

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Not determined

Specificity

This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-Placental alkaline phosphatase (PLAP) [SP15] (ab16695) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trusted by the scientific community
Anti-Placental alkaline phosphatase (PLAP) [SP15] (ab16695) was first used in a scientific publication in 2005 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [SP15] also available for your convenience: ab16695, Carrier free - ab218848, ab238800, Alexa Fluor® 488 - ab270195

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e12413 PubMed38353485

2024

Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk.

Applications

Unspecified application

Species

Unspecified reactive species

Miira M Klemetti,Ante B V Pettersson,Aafaque Ahmad Khan,Leonardo Ermini,Tyler R Porter,Michael L Litvack,Sruthi Alahari,Stacy Zamudio,Nicholas P Illsley,Hannes Röst,Martin Post,Isabella Caniggia

Stem cell research & therapy 12:64 PubMed33461605

2021

The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

You Zhou,Siyu Liu,Wei Wang,Qiang Sun,Mengzhu Lv,Shude Yang,Shuang Tong,Shu Guo

The Journal of biological chemistry 295:16393-16410 PubMed32967966

2020

Calnexin mediates the maturation of GPI-anchors through ER retention.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Guo,Yi-Shi Liu,Xiao-Dong Gao,Taroh Kinoshita,Morihisa Fujita

Genesis (New York, N.Y. : 2000) 58:e23359 PubMed32191380

2020

Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain.

Applications

Unspecified application

Species

Unspecified reactive species

Victoire Cardot-Ruffino,Véronique Chauvet,Cassandre Caligaris,Adrien Bertrand-Chapel,Nicolas Chuvin,Roxane M Pommier,Ulrich Valcourt,David Vincent,Sylvie Martel,Sophie Aires,Bastien Kaniewski,Pierre Dubus,Philippe Cassier,Stéphanie Sentis,Laurent Bartholin

Cells, tissues, organs 207:97-113 PubMed31655811

2019

Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel N Bracey,Alexander H Jinnah,Jeffrey S Willey,Thorsten M Seyler,Ian D Hutchinson,Patrick W Whitlock,Thomas L Smith,Kerry A Danelson,Cynthia L Emory,Bethany A Kerr

eLife 8: PubMed30714906

2019

Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity.

Applications

Unspecified application

Species

Unspecified reactive species

James Ashley,Violet Sorrentino,Meike Lobb-Rabe,Sonal Nagarkar-Jaiswal,Liming Tan,Shuwa Xu,Qi Xiao,Kai Zinn,Robert A Carrillo

Molecular carcinogenesis 58:621-626 PubMed30582228

2018

cd44 deletion suppresses atypia in the precancerous mouse testis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaibiao Li,Shalmali Shukla,Lucien Frappart,Peter Herrlich,Aspasia Ploubidou

Retrovirology 10:4 PubMed23305456

2013

Virion stiffness regulates immature HIV-1 entry.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Bo Pang,Liron Hevroni,Nitzan Kol,Debra M Eckert,Marianna Tsvitov,Michael S Kay,Itay Rousso

The Journal of biological chemistry 287:32525-34 PubMed22851169

2012

Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.

Applications

Species

Unspecified reactive species

Frederike Schmid,Ralf Fliegert,Tim Westphal,Andreas Bauche,Andreas H Guse

Journal of andrology 33:505-13 PubMed21868749

2011

Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice.

Applications

IHC-P

Species

Mouse

Camelia M Saffarini,Nicholas E Heger,Hideki Yamasaki,Tao Liu,Susan J Hall,Kim Boekelheide

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise