JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB129063

Anti-PLAGL1 / ZAC antibody [EPR7523]

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Recombinant Monoclonal PLAGL1 / ZAC antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

LOT1, ZAC, PLAGL1, Zinc finger protein PLAGL1, Lost on transformation 1, Pleiomorphic adenoma-like protein 1, Tumor suppressor ZAC, LOT-1

3 Images
Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)

ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by intracellular flow cytometry (red) or a rabbit IgG negative (green).

Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)
  • WB

Unknown

Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)

All lanes:

Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7523

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Acts as a transcriptional activator (PubMed : 9722527). Involved in the transcriptional regulation of type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
See full target information PLAGL1

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70130 PubMed39365284

2024

PLAGL1 overexpression induces cytoplasmic DNA accumulation that triggers cGAS/STING activation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Li,Lingyan Qiao,Juan Ge,Sicui Hu,Hongxiu Yang,Conghui Hu,Tang Li

Journal of biomedical science 24:55 PubMed28810862

2017

The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Wen Chu,Shu-Ting Liu,Ya-Lan Yang,Shih-Ming Huang,Wei-Ming Wang

Anticancer research 36:617-24 PubMed26851016

2016

PLAGL1 (ZAC1/LOT1) Expression in Clear Cell Renal Cell Carcinoma: Correlations with Disease Progression and Unfavorable Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Janusz Godlewski,Bartlomiej E Krazinski,Anna E Kowalczyk,Jolanta Kiewisz,Jacek Kiezun,Przemyslaw Kwiatkowski,Agnieszka Sliwinska-Jewsiewicka,Zbigniew Maslowski,Zbigniew Kmiec

International journal of oncology 47:951-62 PubMed26134521

2015

Altered expression of the PLAGL1 (ZAC1/LOT1) gene in colorectal cancer: Correlations to the clinicopathological parameters.

Applications

Unspecified application

Species

Unspecified reactive species

Anna E Kowalczyk,Bartlomiej E Krazinski,Janusz Godlewski,Jolanta Kiewisz,Przemyslaw Kwiatkowski,Agnieszka Sliwinska-Jewsiewicka,Jacek Kiezun,Piotr M Wierzbicki,Gabriel Bodek,Marian Sulik,Zbigniew Kmiec

Folia histochemica et cytobiologica 53:96-104 PubMed25823562

2015

PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Janusz Godlewski,Bartlomiej E Krazinski,Jacek Kiezun,Przemyslaw Kwiatkowski,Marian Sulik,Michal Tenderenda,Wojciech Biernat,Zbigniew Kmiec

Human molecular genetics 23:6275-85 PubMed24993786

2014

Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Iglesias-Platas,Alex Martin-Trujillo,Paolo Petazzi,Amy Guillaumet-Adkins,Manel Esteller,David Monk
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com