Rabbit Recombinant Monoclonal PLAGL1 / ZAC antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--western-blot-img107950.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)")
![Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--flow-cytometry-intracellular-img55929.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)")
![OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--oi-rd-scanning-img95297.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (AB129063)")
Publications
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- Journal of biomedical science 24:552017The cytotoxic mechanism of epigallocatechin gallate on proliferative HaCaT keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Wen Chu et. al.PubMed 28810862
- Anticancer research 36:617-242016PLAGL1 (ZAC1/LOT1) Expression in Clear Cell Renal Cell Carcinoma: Correlations with Disease Progression and Unfavorable Prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanusz Godlewski,Bartlomiej E Krazinski,Anna E Kowalczyk,Jolanta Kiewisz,Jacek Kiezun,Przemyslaw Kwiatkowski,Agnieszka Sliwinska-Jewsiewicka,Zbigniew Maslowski,Zbigniew KmiecPubMed 26851016
- International journal of oncology 47:951-622015Altered expression of the PLAGL1 (ZAC1/LOT1) gene in colorectal cancer: Correlations to the clinicopathological parameters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna E Kowalczyk et. al.PubMed 26134521
- Folia histochemica et cytobiologica 53:96-1042015PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanusz Godlewski et. al.PubMed 25823562
- Human molecular genetics 23:6275-852014Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Iglesias-Platas et. al.PubMed 24993786
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Acts as a transcriptional activator (PubMed:9722527). Involved in the transcriptional regulation of type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
Alternative names
LOT1, ZAC, PLAGL1, Zinc finger protein PLAGL1, Lost on transformation 1, Pleiomorphic adenoma-like protein 1, Tumor suppressor ZAC, LOT-1
Recommended products
Rabbit Recombinant Monoclonal PLAGL1 / ZAC antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7523
- Purity
- Tissue culture supernatant
- Dissociation constant
- 1.71 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--western-blot-img107950.jpg "Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)")
Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)
All lanes: Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063) at 1/10000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: Human fetal kidney tissue lysate at 10 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Lane 5: SH-SY5Y cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--flow-cytometry-intracellular-img55929.jpg "Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)")
Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)
ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by intracellular flow cytometry (red) or a rabbit IgG negative (green).
![OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063), expandable thumbnail](https://content.abcam.com/products/images/plagl1-zac-antibody-epr7523-ab129063--oi-rd-scanning-img95297.jpg "OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)")
OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] (ab129063)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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