Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (AB248273)

This data was developed using ab129063, the same antibody clone in a different buffer formulation.

ab129063 at 1/10 dilution staining PLAGL1 / ZAC in permeabilized HepG2 cells by intracellular flow cytometry (red) or a rabbit IgG negative (green).

• WB

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Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (AB248273)

This data was developed using ab129063, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLAGL1 / ZAC antibody [EPR7523] (<a href='/en-us/products/primary-antibodies/plagl1-zac-antibody-epr7523-ab129063'>ab129063</a>) at 1/10000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-PLAGL1 / ZAC antibody [EPR7523] - BSA and Azide free (AB248273)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.