AB189323

Anti-Plakophilin 2/PKP2 antibody

Name: Anti-Plakophilin 2/PKP2 antibody (ab189323) | Abcam
Brand: Abcam Limited
SKU: AB189323
Price: 630 USD
Availability: InStock

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(6 Publications)

Goat Polyclonal Plakophilin 2/PKP2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PKP2 aa 100-150.

View Alternative Names

Plakophilin-2, PKP2

2 Images

Supplier Data

Western blot - Anti-Plakophilin 2/PKP2 antibody (AB189323)

All lanes:

Western blot - Anti-Plakophilin 2/PKP2 antibody (ab189323) at 0.3 µg/mL

All lanes:

Mouse heart lysate at 35 µg

Predicted band size: 97 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 2/PKP2 antibody (AB189323)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 2/PKP2 antibody (AB189323)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling Plakophilin 2/PKP2 with ab189323 at 3.75 μg/ml.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PKP2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q99959

Specificity

ab189323 is expected to recognize both reported isoforms (NP_001005242.2; NP_004563.2).

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plakophilin 2 also known as PKP2 is a protein integral to the function of cell junctions specifically within desmosomes. It is one of the plakophilins a subgroup of the armadillo family proteins. The protein has an approximate molecular weight of 97 kDa. PKP2 is extensively expressed in tissues where cells require strong mechanical adhesion such as cardiac and epidermal tissues. It plays an essential role by anchoring intermediate filaments contributing to structural integrity.

Biological function summary

PKP2 participates in forming and maintaining stable desmosomal connections in cells. It operates as part of the desmosome complex binding to cadherins and linking them to cytoskeletal filaments. This role is important for maintaining cell-cell adhesion during tissue stress particularly in tissues subject to mechanical stress such as the heart. Its interaction with cadherins and intermediate filaments highlights its role in managing cellular integrity and communication.

Pathways

PKP2 is fundamentally involved in cell signaling and junction-related pathways. It forms part of the desmosomal complex pathway which ensures robust cell adhesion and signaling integrity. PKP2 interacts with other proteins in the pathway such as desmoplakin and plakoglobin aiding mechanical stability and signal transduction necessary for tissue integrity and repair.

PKP2 mutations are closely associated with arrhythmogenic right ventricular cardiomyopathy (ARVC) a disease impacting cardiac function. The disorder links PKP2 to desmosomal dysfunction affecting heart muscle integrity and resulting in arrhythmias. Furthermore in the context of skin disorders PKP2 interactions with other desmosomal proteins like desmoglein might influence conditions involving epidermal fragility or blistering.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 25208567). Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins (PubMed : 23884246). Required to maintain gingival epithelial barrier function (PubMed : 34368962). Important component of the desmosome that is also required for localization of desmosome component proteins such as DSC2, DSG2 and JUP to the desmosome cell-cell junction (PubMed : 22781308, PubMed : 25208567). Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls (By similarity). Loss of desmosome cell junctions leads to mis-localization of DSP and DSG2 resulting in disruption of cell-cell adhesion and disordered intermediate filaments (By similarity). Modulates profibrotic gene expression in cardiomyocytes via regulation of DSP expression and subsequent activation of downstream TGFB1 and MAPK14/p38 MAPK signaling (By similarity). Required for cardiac sodium current propagation and electrical synchrony in cardiac myocytes, via ANK3 stabilization and modulation of SCN5A/Nav1.5 localization to cell-cell junctions (By similarity). Required for mitochondrial function, nuclear envelope integrity and positive regulation of SIRT3 transcription via maintaining DES localization at its nuclear envelope and cell tip anchoring points, and thereby preserving regulation of the transcriptional program (PubMed : 35959657). Maintenance of nuclear envelope integrity protects against DNA damage and transcriptional dysregulation of genes, especially those involved in the electron transport chain, thereby preserving mitochondrial function and protecting against superoxide radical anion generation (PubMed : 35959657). Binds single-stranded DNA (ssDNA) (PubMed : 20613778). Involved in the inhibition of viral infection by influenza A viruses (IAV) (PubMed : 28169297). Acts as a host restriction factor for IAV viral propagation, potentially via disrupting the interaction of IAV polymerase complex proteins (PubMed : 28169297).

See full target information PKP2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1177847 PubMed37583701

2023

Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Shu,Jinni Jiang,Guofang Zhao

International journal of molecular sciences 24: PubMed36768439

2023

Alterations in Calcium Handling Are a Common Feature in an Arrhythmogenic Cardiomyopathy Cell Model Triggered by Desmosome Genes Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vallverdú-Prats,David Carreras,Guillermo J Pérez,Oscar Campuzano,Ramon Brugada,Mireia Alcalde

International journal of molecular sciences 23: PubMed35054841

2022

Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vallverdú-Prats,Ramon Brugada,Mireia Alcalde

Cellular and molecular gastroenterology and hepatology 13:1181-1200 PubMed34929421

2021

Desmoplakin Maintains Transcellular Keratin Scaffolding and Protects From Intestinal Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Gross,Biaohuan Zhou,Lisa Bewersdorf,Nicole Schwarz,Gabriel M Schacht,Peter Boor,Konrad Hoeft,Bernd Hoffmann,Elaine Fuchs,Rafael Kramann,Rudolf Merkel,Rudolf E Leube,Pavel Strnad

Cardiology 145:136-147 PubMed32007997

2020

Cardiac-Specific Caveolin-3 Overexpression Prevents Post-Myocardial Infarction Ventricular Arrhythmias by Inhibiting Ryanodine Receptor-2 Hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Zhang,Qin Fang,Tingyi Du,Guangzhi Chen,Yan Wang,Dao Wen Wang

Molecular medicine reports 20:5064-5074 PubMed31638226

2019

Antitumor effects and mechanisms of 1,25(OH)2D3 in the Pfeiffer diffuse large B lymphoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Han,Yonghong Tang,Meizuo Zhong,Wenlin Wu

View all publications

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Product promise

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Anti-Plakophilin 2/PKP2 antibody

Western blot - Anti-Plakophilin 2/PKP2 antibody (AB189323)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 2/PKP2 antibody (AB189323)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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