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Rabbit Recombinant Monoclonal Plakophilin 3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

May play a role in junctional plaques.

Alternative names

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Rabbit Recombinant Monoclonal Plakophilin 3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR5560

Purification technique

Affinity purification Protein A

Dissociation constant

1.66 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

Plakophilin 3 plays a vital role in stabilizing junctional complexes by anchoring desmosomal components to the cytoskeleton. It usually forms a part of the desmosome a cellular structure composed of desmogleins desmocollins and plakoglobin. Through these interactions PKP3 contributes to the robust mechanical strength of tissue architecture and participates in signaling pathways that regulate cell proliferation and differentiation.

Mechanical summary

Plakophilin 3 often referred to as PKP3 is a member of the Armadillo family of proteins. PKP3 is a desmosomal plaque protein with a known molecular mass of approximately 84 kDa. It localizes primarily to desmosomes and the nucleus where it contributes to cell-cell adhesion. PKP3 is commonly expressed in epithelial tissues where it helps maintain the structural integrity of epithelial sheets by binding to desmosomal cadherins.

Pathway

Plakophilin 3 integrates into desmosome-related signaling pathways notably the intercellular adhesion pathways and the Wnt signaling pathway. PKP3 associates with proteins such as plakoglobin and β-catenin which are also involved in Wnt signaling influencing cell-cell contact and transcriptional regulation. These pathways are important for maintaining the balance between cell adhesion and cellular signaling ensuring appropriate responses to developmental and environmental cues.

Disease

Plakophilin 3 shows a connection to certain forms of cancer including prostate cancer and squamous cell carcinoma. PKP3 expression levels have an association with tumor progression and metastasis. In prostate cancer for instance altered expression of desmosomal components such as PKP3 can influence cancer cell adhesion and mobility. PKP3 interacts with proteins like E-cadherin in these pathological states which can affect adhesion properties and promote malignancy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Plakophilin 3 antibody [EPR5560] (ab109441), expandable thumbnail

    Western blot - Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    False colour image of Western blot: Anti-Plakophilin 3 antibody [EPR5560] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109441 was shown to bind specifically to Plakophilin 3. A band was observed at 70 and 80 kDa in wild-type HeLa cell lysates with no signal observed at this size in PKP3 CRISPR-Cas9 edited cell line ab265539 (CRISPR-Cas9 edited cell lysate ab258120). The band observed in the CRISPR-Cas9 edited lysate lane below 70 and 80 kDa is likely to represent a truncated form of Plakophilin 3. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and PKP3 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Plakophilin 3 antibody [EPR5560] (AB109441) at 1/10000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PKP3 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Predicted band size: 87 kDa

    Observed band size: 70 kDa, 80 kDa

  • Western blot - Anti-Plakophilin 3 antibody [EPR5560] (ab109441), expandable thumbnail

    Western blot - Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    All lanes: Western blot - Anti-Plakophilin 3 antibody [EPR5560] (AB109441) at 1/10000 dilution

    Lane 1: Caco-2 cell lysate at 10 µg

    Lane 2: HACAT cell lysate at 10 µg

    Predicted band size: 87 kDa

    Observed band size: 87 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 3 antibody [EPR5560] (ab109441), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    ab109441 at 1/100 dilution staining Human paraffin-embedded breast tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 3 antibody [EPR5560] (ab109441), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    ab109441 at 1/100 dilution staining paraffin-embedded Human kidney tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Plakophilin 3 antibody [EPR5560] (ab109441), expandable thumbnail

    OI-RD Scanning - Anti-Plakophilin 3 antibody [EPR5560] (ab109441)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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