Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)

Rabbit Recombinant Monoclonal Plasma Kallikrein 1B antibody. Carrier free. Suitable for WB, IP, IHC-P, Dot and reacts with Mouse, Rat, Recombinant fragment - Mouse samples.

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Images

Dot Blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (AB313892), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	IHC-P	Dot	Flow Cyt (Intra)	IHC-Fr	ICC/IF
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended
Rat	Tested	Expected	Tested	Expected	Not recommended	Not recommended	Not recommended
Recombinant fragment - Mouse	Not recommended	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Klkb1

Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Alternative names

Klk3, Pk, Klkb1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein

Recommended products

Rabbit Recombinant Monoclonal Plasma Kallikrein 1B antibody. Carrier free. Suitable for WB, IP, IHC-P, Dot and reacts with Mouse, Rat, Recombinant fragment - Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28280-31
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab313892 is the carrier-free version of Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.

Biological function summary

Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.

Pathways

Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.

Associated diseases and disorders

Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Dot Blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Dot blot analysis of Plasma Kallikrein 1B using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1:1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution. Exposure time: 180 seconds. Blocking and diluting buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Plasma Kallikrein 1B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 IP in Mouse liver tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 in mouse liver tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 180 seconds
All lanes: Immunoprecipitation - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] (Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891) at 1/30 dilution
All lanes: Mouse liver tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: spleen (PMID: 30559188), brain, liver.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30559188, 29489063).

The identity of the lower MW band at approximately 36 kDa is light chain of Plasma Kallikrein 1B.
The bands above 80 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 3.25 seconds
All lanes: Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] (Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891) at 1/1000 dilution
Lane 1: Rat serum lysate at 10 µg
Lane 2: Rat plasma lysate at 10 µg
Lane 3: Rat spleen lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
Lane 5: Rat liver lysate at 40 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 80 kDa
Exposure time: 3.25s
Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.

Low expression: spleen (PMID: 30559188), brain.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30559188, 29489063).
The identity of the lower MW band at approximately 36 kDa is light chain of Plasma Kallikrein 1B.
The bands above 80 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 3.25 seconds
All lanes: Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] (Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891) at 1/1000 dilution
Lane 1: Mouse plasma lysate at 10 µg
Lane 2: Mouse serum lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Mouse brain lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 80 kDa
Exposure time: 3.25s
Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Plasma Kallikrein 1B detected a shift from 80kDa to 75kDa band after deglycosylation treatment.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] (Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891) at 1/1000 dilution
Lane 1: Untreated mouse liver tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate treated with Protein treated with Peptide:N-glycosidase F (PNGase F) at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 80 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on mouse cerebrum. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on rat cerebrum. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Non-perfused fixed r tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on non-perfused fixed rat liver. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma of rat kidney. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Non-perfused fixed m tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on non-perfused fixed mouse liver. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR28280-31] - BSA and Azide free (ab313892)
This data was developed using Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Plasma Kallikrein 1B with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 at 1/500 (1.038 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on plasma of mouse kidney. The section was incubated with Anti-Plasma Kallikrein 1B antibody [EPR28280-31] ab313891 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins