AB154560

Anti-Plasminogen antibody

Name: Anti-Plasminogen antibody (ab154560) | Abcam
Brand: Abcam Limited
SKU: AB154560

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(20 Publications)

Rabbit Polyclonal Plasminogen antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLG aa 50-450.

View Alternative Names

Plasminogen, PLG

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Immunohistochemical analysis of paraffin-embedded human Hepatoma tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody (AB154560)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody (AB154560)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Plasminogen using ab154560 at a 1/500 dilution. Lower image is costained with Hoechst 33342.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft, labeling Plasminogen using ab154560 at a 1/500 dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Immunohistochemical analysis of paraffin-embedded Rat prostate tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.

Unknown

Western blot - Anti-Plasminogen antibody (AB154560)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Lane 3:

K562 whole cell lysate at 30 µg

Predicted band size: 90 kDa

false

Unknown

Western blot - Anti-Plasminogen antibody (AB154560)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution

All lanes:

BCL1 whole cell lysate at 30 µg

Predicted band size: 90 kDa

false

Unknown

Western blot - Anti-Plasminogen antibody (AB154560)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution

Lane 1:

PC12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PLG aa 50-450. The exact immunogen used to generate this antibody is proprietary information.

P00747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.

Biological function summary

Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.

Pathways

Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.

Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1, C4 and C5 (PubMed : 6447255). Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed : 21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed : 21949403).. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.. (Microbial infection) Interacts with dengue virus type 2 particles (PubMed : 31726374). Enhances dengue virus type 2 infection in Aedes aegypti mosquito midgut by increasing midgut internalization, resulting in higher infection rates and viral dissemination in mosquitoes (PubMed : 31726374).

See full target information PLG

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Matrix biology plus 23:100159 PubMed39220302

2024

Matrisome proteomics reveals novel mediators of muscle remodeling with aerobic exercise training.

Applications

Unspecified application

Species

Unspecified reactive species

Pattarawan Pattamaprapanont,Eileen M Cooney,Tara L MacDonald,Joao A Paulo,Hui Pan,Jonathan M Dreyfuss,Sarah J Lessard

Frontiers in pediatrics 12:1338672 PubMed38379911

2024

Activation of renal epithelial Na channels (ENaC) in infants with congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Ortmann,Shyam Nandi,Yu-Long Li,Hong Zheng,Kaushik P Patel

Artificial organs 48:734-742 PubMed38380722

2024

The effect of ultrasound-assisted thrombolysis studied in blood-on-a-chip.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Yongjian Li,Haosheng Chen

International journal of molecular sciences 24: PubMed37958726

2023

A Serine Protease Inhibitor, Camostat Mesilate, Suppresses Urinary Plasmin Activity and Alleviates Hypertension and Podocyte Injury in Dahl Salt-Sensitive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yasunobu Iwata,Qinyuan Deng,Yutaka Kakizoe,Terumasa Nakagawa,Yoshikazu Miyasato,Miyuki Nakagawa,Kayo Nishiguchi,Yu Nagayoshi,Yuki Narita,Yuichiro Izumi,Takashige Kuwabara,Masataka Adachi,Masashi Mukoyama

Journal of diabetes 15:777-786 PubMed37314019

2023

Increased glutamate in type 2 diabetes in the Korean population is associated with increased plasminogen levels.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo Jung Lee,Jeong Won Yeom,Ji Ho Yun,Han Byul Jang,Min-Gyu Yoo,Hyo-Jin Kim,Soo Kyung Koo,Hye-Ja Lee

Iranian journal of basic medical sciences 25:715-722 PubMed35949311

2022

Analysis of transcriptome characteristics of UTI therapy for cerebral injury after CA/ROSC based on RNA-seq technique.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Bai,Mingya Yang,Tiantian Zhu,Jun Xu,Wei Wang,Yuanyuan He,Yu Liu,Xingxing Li,Miao He,Tao Meng,Zhenzhen Wang,Hong Zhang,Lixin Zhu

Nutrients 14: PubMed35684129

2022

New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation.

Applications

Unspecified application

Species

Unspecified reactive species

Po-An Hu,Sz-Han Wang,Chia-Hui Chen,Bei-Chia Guo,Jenq-Wen Huang,Tzong-Shyuan Lee

Molecular oncology 16:2214-2234 PubMed34861103

2021

Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eun Yu,In Jun Yeo,Dong Ju Son,Jaesuk Yun,Sang-Bae Han,Jin Tae Hong

Cell chemical biology 28:1728-1739.e5 PubMed34352225

2021

The role of SERPIN citrullination in thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ronak Tilvawala,Venkatesh V Nemmara,Archie C Reyes,Nicoletta Sorvillo,Ari J Salinger,Deya Cherpokova,Saeko Fukui,Sarah Gutch,Denisa Wagner,Paul R Thompson

Journal of the American Heart Association 9:e016387 PubMed33215566

2020

Plasminogen Deficiency and Amiloride Mitigate Angiotensin II-Induced Hypertension in Type 1 Diabetic Mice Suggesting Effects Through the Epithelial Sodium Channel.

Applications

Unspecified application

Species

Unspecified reactive species

Henrik Andersen,Maria Høj Hansen,Kristian B Buhl,Mette Stæhr,Ulla G Friis,Camilla Enggaard,Shanya Supramaniyam,Ida K Lund,Per Svenningsen,Pernille B L Hansen,Boye L Jensen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plasminogen antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody (AB154560)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB154560)

Western blot - Anti-Plasminogen antibody (AB154560)

Western blot - Anti-Plasminogen antibody (AB154560)

Western blot - Anti-Plasminogen antibody (AB154560)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise