Rabbit Polyclonal Plasminogen antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLG aa 50-450.
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- International journal of molecular sciences 24:2023A Serine Protease Inhibitor, Camostat Mesilate, Suppresses Urinary Plasmin Activity and Alleviates Hypertension and Podocyte Injury in Dahl Salt-Sensitive Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasunobu Iwata,Qinyuan Deng,Yutaka Kakizoe,Terumasa Nakagawa,Yoshikazu Miyasato,Miyuki Nakagawa,Kayo Nishiguchi,Yu Nagayoshi,Yuki Narita,Yuichiro Izumi,Takashige Kuwabara,Masataka Adachi,Masashi MukoyamaPubMed 37958726
- Iranian journal of basic medical sciences 25:715-7222022Analysis of transcriptome characteristics of UTI therapy for cerebral injury after CA/ROSC based on RNA-seq technique.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojie Bai,Mingya Yang,Tiantian Zhu,Jun Xu,Wei Wang,Yuanyuan He,Yu Liu,Xingxing Li,Miao He,Tao Meng,Zhenzhen Wang,Hong Zhang,Lixin ZhuPubMed 35949311
- Nutrients 14:2022New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPo-An Hu,Sz-Han Wang,Chia-Hui Chen,Bei-Chia Guo,Jenq-Wen Huang,Tzong-Shyuan LeePubMed 35684129
- Molecular oncology 16:2214-22342021Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Eun Yu,In Jun Yeo,Dong Ju Son,Jaesuk Yun,Sang-Bae Han,Jin Tae HongPubMed 34861103
- Cell chemical biology 28:1728-1739.e52021The role of SERPIN citrullination in thrombosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonak Tilvawala,Venkatesh V Nemmara,Archie C Reyes,Nicoletta Sorvillo,Ari J Salinger,Deya Cherpokova,Saeko Fukui,Sarah Gutch,Denisa Wagner,Paul R ThompsonPubMed 34352225
- Journal of the American Heart Association 9:e0163872020Plasminogen Deficiency and Amiloride Mitigate Angiotensin II-Induced Hypertension in Type 1 Diabetic Mice Suggesting Effects Through the Epithelial Sodium Channel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenrik Andersen et. al.PubMed 33215566
- Molecular psychiatry 26:6023-60372020The human brain NGF metabolic pathway is impaired in the pre-clinical and clinical continuum of Alzheimers disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRowan Pentz,M Florencia Iulita,Adriana Ducatenzeiler,David A Bennett,A Claudio CuelloPubMed 32488129
- Acta physiologica (Oxford, England) 231:e135122020Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyun Xiao,Bernhard N Bohnert,Hande Aypek,Oliver Kretz,Florian Grahammer,Ute Aukschun,Matthias Wörn,Andrea Janessa,Daniel Essigke,Christoph Daniel,Kerstin Amann,Tobias B Huber,Edward F Plow,Andreas L Birkenfeld,Ferruh ArtuncPubMed 32455507
- Acta physiologica (Oxford, England) 227:e132862019Urokinase-type plasminogen activator (uPA) is not essential for epithelial sodium channel (ENaC)-mediated sodium retention in experimental nephrotic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBernhard N Bohnert,Sophie Daiminger,Matthias Wörn,Florian Sure,Tobias Staudner,Alexandr V Ilyaskin,Firas Batbouta,Andrea Janessa,Jonas C Schneider,Daniel Essigke,Sandip Kanse,Silke Haerteis,Christoph Korbmacher,Ferruh ArtuncPubMed 31006168
- Journal of molecular medicine (Berlin, Germany) 96:1333-13442018Plasminogen/thrombomodulin signaling enhances VEGF expression to promote cutaneous wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsung-Lin Cheng et. al.PubMed 30341568
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLG aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link P00747
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed:21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed:21949403). (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.
Alternative names
Plasminogen, PLG
Recommended products
Rabbit Polyclonal Plasminogen antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLG aa 50-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLG aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link P00747
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
- Biological function summary
Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.
- Pathways
Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.
- Associated diseases and disorders
Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-Plasminogen antibody (ab154560)
7.5% SDS PAGE
All lanes: Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution
All lanes: BCL1 whole cell lysate at 30 µg
Predicted band size: 90 kDa
Western blot - Anti-Plasminogen antibody (ab154560)
7.5% SDS PAGE
All lanes: Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: K562 whole cell lysate at 30 µg
Predicted band size: 90 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (ab154560)
Immunohistochemical analysis of paraffin-embedded Rat prostate tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.
Western blot - Anti-Plasminogen antibody (ab154560)
7.5% SDS PAGE
All lanes: Western blot - Anti-Plasminogen antibody (ab154560) at 1/1000 dilution
Lane 1: PC12 whole cell lysate at 30 µg
Lane 2: Rat2 whole cell lysate at 30 µg
Predicted band size: 90 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody (ab154560)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Plasminogen using ab154560 at a 1/500 dilution. Lower image is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (ab154560)
Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft, labeling Plasminogen using ab154560 at a 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (ab154560)
Immunohistochemical analysis of paraffin-embedded human Hepatoma tissue, labeling Plasminogen using ab154560 at a 1/500 dilution.
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