Anti-Plasminogen antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB180046)

Paraffin embedded human placenta tissue stained for Plasminogen using ab180046 at 1 µg/ml in immunohistochemical analysis. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody (AB180046)

Paraffin embedded human mammary cancer tissue stained for Plasminogen using ab180046 at 1 μg/ml in immunohistochemical analysis. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 minutes. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• WB

Supplier Data

Western blot - Anti-Plasminogen antibody (AB180046)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature. The membrane was incubated with primary antibody overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with secondary antibody for 1.5 hour at room temperature.

All lanes:

Western blot - Anti-Plasminogen antibody (ab180046) at 0.5 µg/mL

Lane 1:

SMMC-7721 (human hepatocarcinoma cell line) whole cell lysate at 50 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 90 kDa

Observed band size: 75 kDa,95 kDa

true

• WB

Supplier Data

Western blot - Anti-Plasminogen antibody (AB180046)

5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes.

All lanes:

Western blot - Anti-Plasminogen antibody (ab180046) at 0.5 µg/mL

Lane 1:

human placenta tissue lysates at 30 µg

Lane 2:

human CACO-2 whole cell lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP antibody at 1/5000 dilution

true