AB308224

Anti-Plasminogen antibody [HL2071]

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Plasminogen antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse, Zebrafish, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Rhesus monkey PLG.

View Alternative Names

Plasminogen, PLG

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (AB308224)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed HeLa cells labelling Plasminogen with ab308224 at 1/250 dilution at RT for 15 min.

Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000 dilution.

Blue : Fluoroshield with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling Plasminogen with ab308224 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Paraffin-embedded rat kidney tissue stained for Plasminogen protein at cell membrane and secreted with ab308224 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Plasminogen antibody [HL2071] (AB308224)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Plasminogen antibody [HL2071] (ab308224) at 1/1000 dilution

Lane 1:

Human plasma at 30 µg

Lane 2:

Mouse plasma at 30 µg

Lane 3:

Rat plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 90 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunohistochemical analysis of paraffin-embedded cat liver tissue labelling Plasminogen with ab308224 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Plasminogen antibody [HL2071] (AB308224)

The signal was developed with Trident ECL plus-Enhanced

All lanes:

Western blot - Anti-Plasminogen antibody [HL2071] (ab308224) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2071

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human, Rat, Cat, Zebrafish

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rhesus monkey PLG. The exact immunogen used to generate this antibody is proprietary information.

P12545

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiostatin a fragment derived from plasminogen acts critically in inhibiting angiogenesis. It has a molecular mass of approximately 38 kDa. Angiostatin primarily derives from proteolytic cleavage of plasminogen within the vascular system. Expression of angiostatin occurs in various tissues but it is predominantly observed around tumor sites where it exerts its anti-angiogenic effects.

Biological function summary

Angiostatin functions to inhibit the proliferation and migration of endothelial cells thereby blocking new blood vessel formation. It achieves this by binding to ATP synthase on the surface of endothelial cells. This protein operates as an isolated entity and does not typically form part of a larger complex. Angiostatin’s activity is essential in controlling physiological processes requiring reduced blood vessel development.

Pathways

Angiostatin participates actively in the angiogenesis pathway interacting with factors like vascular endothelial growth factor (VEGF). It opposes pro-angiogenic signals by suppressing these pathways reducing the available nutrients to target tissues such as tumors. Angiostatin's role in the lysine-binding pathway also connects it to proteins like plasmin influencing fibrinolytic activity.

Angiostatin associates strongly with cancer and ocular diseases like diabetic retinopathy. Its inhibitory effect on angiogenesis restricts the blood supply to tumors aiding in cancer treatment. In cancer angiostatin interacts with plasminogen activator inhibitor-1 (PAI-1) to modulate plasminogen activity. In ocular diseases angiostatin’s impact on abnormal blood vessel growth contributes to therapeutic strategies to manage these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1, C4 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).

See full target information PLG

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plasminogen antibody [HL2071]

Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Western blot - Anti-Plasminogen antibody [HL2071] (AB308224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224)

Western blot - Anti-Plasminogen antibody [HL2071] (AB308224)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise