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Rabbit Monoclonal Plasminogen antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse, Zebrafish, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Rhesus monkey PLG.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224), expandable thumbnail
  • Western blot - Anti-Plasminogen antibody [HL2071] (AB308224), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (AB308224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (AB308224), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Rhesus monkey PLG. The exact immunogen used to generate this antibody is proprietary information. Database link P12545

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Expected
Tested
Mouse
Tested
Tested
Expected
Rat
Tested
Tested
Expected
Cat
Expected
Tested
Expected
Zebrafish
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Zebrafish
Dilution info
1/500 - 1/3000
Notes

-

Expected
Expected

Species
Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Cat, Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Zebrafish
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse, Zebrafish, Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).

Alternative names

Plasminogen, PLG

Recommended products

Rabbit Monoclonal Plasminogen antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse, Zebrafish, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Rhesus monkey PLG.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Rhesus monkey PLG. The exact immunogen used to generate this antibody is proprietary information. Database link P12545
Carrier free
Yes
Clone number
HL2071
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Angiostatin a fragment derived from plasminogen acts critically in inhibiting angiogenesis. It has a molecular mass of approximately 38 kDa. Angiostatin primarily derives from proteolytic cleavage of plasminogen within the vascular system. Expression of angiostatin occurs in various tissues but it is predominantly observed around tumor sites where it exerts its anti-angiogenic effects.

Biological function summary

Angiostatin functions to inhibit the proliferation and migration of endothelial cells thereby blocking new blood vessel formation. It achieves this by binding to ATP synthase on the surface of endothelial cells. This protein operates as an isolated entity and does not typically form part of a larger complex. Angiostatin’s activity is essential in controlling physiological processes requiring reduced blood vessel development.

Pathways

Angiostatin participates actively in the angiogenesis pathway interacting with factors like vascular endothelial growth factor (VEGF). It opposes pro-angiogenic signals by suppressing these pathways reducing the available nutrients to target tissues such as tumors. Angiostatin's role in the lysine-binding pathway also connects it to proteins like plasmin influencing fibrinolytic activity.

Associated diseases and disorders

Angiostatin associates strongly with cancer and ocular diseases like diabetic retinopathy. Its inhibitory effect on angiogenesis restricts the blood supply to tumors aiding in cancer treatment. In cancer angiostatin interacts with plasminogen activator inhibitor-1 (PAI-1) to modulate plasminogen activity. In ocular diseases angiostatin’s impact on abnormal blood vessel growth contributes to therapeutic strategies to manage these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224)

    Paraffin-embedded rat kidney tissue stained for Plasminogen protein at cell membrane and secreted with ab308224 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Western blot - Anti-Plasminogen antibody [HL2071] (ab308224)

    Samples were seperated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-Plasminogen antibody [HL2071] (ab308224) at 1/1000 dilution

    Lane 1: Human plasma at 30 µg

    Lane 2: Mouse plasma at 30 µg

    Lane 3: Rat plasma at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Plasminogen antibody [HL2071] (ab308224)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed HeLa cells labelling Plasminogen with ab308224 at 1/250 dilution at RT for 15 min.

    Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000 dilution.

    Blue: Fluoroshield with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224)

    Immunohistochemical analysis of paraffin-embedded cat liver tissue labelling Plasminogen with ab308224 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [HL2071] (ab308224)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling Plasminogen with ab308224 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Western blot - Anti-Plasminogen antibody [HL2071] (ab308224)

    The signal was developed with Trident ECL plus-Enhanced

    All lanes: Western blot - Anti-Plasminogen antibody [HL2071] (ab308224) at 1/1000 dilution

    All lanes: Whole zebrafish extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 44 kDa

  • Western blot - Anti-Plasminogen antibody [HL2071] (ab308224), expandable thumbnail

    Western blot - Anti-Plasminogen antibody [HL2071] (ab308224)

    Human plasma, mouse plasma and rat plasma were separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody [HL2071] (ab308224) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: Human plasma lysate

    Lane 2: Mouse plasma lysate

    Lane 3: Rat plasma lysate

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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