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Rabbit Polyclonal Plastin L antibody. Suitable for IHC-P, WB and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish Plastin-2 aa 50-450.


Images

Western blot - Anti-Plastin L antibody (AB210099), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody (AB210099), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Zebrafish Plastin-2 aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P698

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Zebrafish
Tested
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/100 - 1/1000

Notes

Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) 95°C, 1 Hour

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Zebrafish

Dilution info

1/500 - 1/3000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Actin-binding protein. Plays a role in the activation of T-cells (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Plastin L antibody. Suitable for IHC-P, WB and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish Plastin-2 aa 50-450.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Zebrafish Plastin-2 aa 50-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P698
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Plastin L also known as L-Plastin or plastine is a type of actin-bundling protein involved in regulating cytoskeletal rearrangements. This protein has a mass of approximately 70 kDa and serves an essential role in maintaining cell structure and mobility by cross-linking actin filaments. It is predominantly found in hematopoietic cells such as leukocytes but also expressed in certain non-hematopoietic cells. In zebrafish studies plastin L has been important for understanding its conservation and function across species.

Biological function summary

Plastin L participates in cellular processes such as phagocytosis cell motility and immune responses by stabilizing actin filaments. It does not form part of a larger protein complex but interacts with actin to perform its functions. As an actin-binding protein it helps in the formation and maintenance of the cytoskeleton impacting the cell's mechanical properties and response to external stimuli.

Pathways

Plastin L plays significant roles in immune cell signaling and cytoskeletal rearrangements. It is involved in pathways such as the immune response and cell adhesion where it contributes to leukocyte emigration and inflammation. Plastin L is functionally related to proteins like talin and paxillin which are key in mediating signal transduction resulting in cytoskeletal remodeling.

Associated diseases and disorders

Plastin L is associated with cancer and immune system disorders. Overexpression of plastin L correlates with various forms of cancer highlighting its role in tumor cell invasion and metastasis. Additionally it shows a connection with autoimmune conditions where deregulation of actin polymerization affects immune cell function. In these contexts proteins like cofilin interact with plastin L to modulate actin dynamics and cellular responses.

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2 product images

  • Western blot - Anti-Plastin L antibody (ab210099), expandable thumbnail

    Western blot - Anti-Plastin L antibody (ab210099)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-Plastin L antibody (ab210099) at 1/1000 dilution

    All lanes: Zebrafish kidney whole cell lysate at 30 µg

    Predicted band size: 61 kDa, 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody (ab210099), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody (ab210099)

    Immunohistochemical analysis of paraffin embedded Zebrafish tissue, labeling Plastin L using ab210099 at 1/300 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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