Anti-Plastin L antibody [EPR4277]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

ab109124 at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Rat testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control : Negligible staining on Rat testis.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Mouse liver labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control : Negligible staining on mouse liver.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control : no staining on the cardiac muscle in mouse heart, but staining the stroma immune cells.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Mouse spleen labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse spleen. The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Mouse testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control : Negligible staining on mouse testis.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (AB109124)

Immunohistochemical analysis of paraffin-embedded Mouse colon labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the immune cells in mouse colon. The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Unknown

Western blot - Anti-Plastin L antibody [EPR4277] (AB109124)

All lanes:

Western blot - Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Lane 1:

THP1 cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 70 kDa

false

• WB

Supplier Data

Western blot - Anti-Plastin L antibody [EPR4277] (AB109124)

Blocking and dilution buffer : 5% NFDM/TBST.

Low expression control : rat brain, rat liver, rat testis.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Lane 1:

Low expression control: Rat brain tissue lysate at 20 µg

Lane 2:

Low expression control: Rat liver tissue lysate at 20 µg

Lane 3:

Low expression control: Rat testis tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Observed band size: 80 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Plastin L antibody [EPR4277] (AB109124)

Blocking and dilution buffer : 5% NFDM/TBST.

Low expression control : mouse brain, mouse liver, mouse testis.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Observed band size: 70 kDa

false

Exposure time: 10s