Rabbit Recombinant Monoclonal Plastin L antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img32982.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47902.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432179.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432177.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (AB247772)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Tested |
| Rat | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Actin-binding protein (PubMed:16636079, PubMed:17294403, PubMed:28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed:17294403).
Alternative names
Plastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1, LCP1, PLS2
Recommended products
Rabbit Recombinant Monoclonal Plastin L antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Plastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1, LCP1, PLS2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4277
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247772 is the carrier-free version of Anti-Plastin L antibody [EPR4277] ab109124.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plastin L also known as L-Plastin or plastine is a type of actin-bundling protein involved in regulating cytoskeletal rearrangements. This protein has a mass of approximately 70 kDa and serves an essential role in maintaining cell structure and mobility by cross-linking actin filaments. It is predominantly found in hematopoietic cells such as leukocytes but also expressed in certain non-hematopoietic cells. In zebrafish studies plastin L has been important for understanding its conservation and function across species.
- Biological function summary
Plastin L participates in cellular processes such as phagocytosis cell motility and immune responses by stabilizing actin filaments. It does not form part of a larger protein complex but interacts with actin to perform its functions. As an actin-binding protein it helps in the formation and maintenance of the cytoskeleton impacting the cell's mechanical properties and response to external stimuli.
- Pathways
Plastin L plays significant roles in immune cell signaling and cytoskeletal rearrangements. It is involved in pathways such as the immune response and cell adhesion where it contributes to leukocyte emigration and inflammation. Plastin L is functionally related to proteins like talin and paxillin which are key in mediating signal transduction resulting in cytoskeletal remodeling.
- Associated diseases and disorders
Plastin L is associated with cancer and immune system disorders. Overexpression of plastin L correlates with various forms of cancer highlighting its role in tumor cell invasion and metastasis. Additionally it shows a connection with autoimmune conditions where deregulation of actin polymerization affects immune cell function. In these contexts proteins like cofilin interact with plastin L to modulate actin dynamics and cellular responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img32982.jpg "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Plastin L antibody [EPR4277] (Anti-Plastin L antibody [EPR4277] ab109124) at 1/1000 dilution
Lane 1: THP1 cell lysates at 10 µg
Lane 2: K562 cell lysates at 10 µg
Lane 3: Jurkat cell lysates at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47902.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.Anti-Plastin L antibody [EPR4277] ab109124, at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the mouse spleen. The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432179.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression control: Negligible staining on Rat testis.
The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432177.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression control: Negligible staining on mouse testis.
The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432176.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression control: no staining on the cardiac muscle in mouse heart, but staining the stroma immune cells.
The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432178.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression control: Negligible staining on mouse liver.
The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img432165.jpg "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: mouse brain, mouse liver, mouse testis.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-Plastin L antibody [EPR4277] (Anti-Plastin L antibody [EPR4277] ab109124) at 1/1000 dilution
Lane 1: Low expression control: Rat brain tissue lysate at 20 µg
Lane 2: Low expression control: Rat liver tissue lysate at 20 µg
Lane 3: Low expression control: Rat testis tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Observed band size: 80 kDa
Exposure time: 10s
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: mouse brain, mouse liver, mouse testis.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img432164.jpg "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: mouse brain, mouse liver, mouse testis.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-Plastin L antibody [EPR4277] (Anti-Plastin L antibody [EPR4277] ab109124) at 1/1000 dilution
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Observed band size: 70 kDa
Exposure time: 10s
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: mouse brain, mouse liver, mouse testis.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432173.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the immune cells in mouse colon. The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432175.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
This data was developed using Anti-Plastin L antibody [EPR4277] ab109124, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen labelling Plastin L with Anti-Plastin L antibody [EPR4277] ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the rat spleen. The section was incubated with Anti-Plastin L antibody [EPR4277] ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432176.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432178.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img432165.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
![Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--western-blot-img432164.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772), expandable thumbnail](https://content.abcam.com/products/images/plastin-l-antibody-epr4277-bsa-and-azide-free-ab247772--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432173.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)")
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