AB247773

Anti-Plastin L antibody [EPR4278] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Plastin L antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PLS2, LCP1, Plastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1

3 Images

Flow Cytometry (Intracellular) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

This data was developed using ab109129, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) cells labeling Plastin L with purified ab109129 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

This data was developed using ab109129, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Plastin L antibody [EPR4278] (<a href='/en-us/products/primary-antibodies/plastin-l-antibody-epr4278-ab109129'>ab109129</a>) at 1/10000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Predicted band size: 70 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

This data was developed using ab109129, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab109129 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4278

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab247773 is the carrier-free version of ab109129.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plastin L also known as L-Plastin or plastine is a type of actin-bundling protein involved in regulating cytoskeletal rearrangements. This protein has a mass of approximately 70 kDa and serves an essential role in maintaining cell structure and mobility by cross-linking actin filaments. It is predominantly found in hematopoietic cells such as leukocytes but also expressed in certain non-hematopoietic cells. In zebrafish studies plastin L has been important for understanding its conservation and function across species.

Biological function summary

Plastin L participates in cellular processes such as phagocytosis cell motility and immune responses by stabilizing actin filaments. It does not form part of a larger protein complex but interacts with actin to perform its functions. As an actin-binding protein it helps in the formation and maintenance of the cytoskeleton impacting the cell's mechanical properties and response to external stimuli.

Pathways

Plastin L plays significant roles in immune cell signaling and cytoskeletal rearrangements. It is involved in pathways such as the immune response and cell adhesion where it contributes to leukocyte emigration and inflammation. Plastin L is functionally related to proteins like talin and paxillin which are key in mediating signal transduction resulting in cytoskeletal remodeling.

Plastin L is associated with cancer and immune system disorders. Overexpression of plastin L correlates with various forms of cancer highlighting its role in tumor cell invasion and metastasis. Additionally it shows a connection with autoimmune conditions where deregulation of actin polymerization affects immune cell function. In these contexts proteins like cofilin interact with plastin L to modulate actin dynamics and cellular responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein (PubMed : 16636079, PubMed : 17294403, PubMed : 28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed : 17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed : 17294403).

See full target information LCP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plastin L antibody [EPR4278] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

Western blot - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4278] - BSA and Azide free (AB247773)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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