Anti-PLBD2 antibody [EPR21204]

2 product images

• 

  Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA solution before being incubated with ab225580 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-PLBD2 antibody [EPR21204] (ab225580) at 1 µg/mL

  Lane 1: Human heart tissue lysate at 10 µg

  Lane 2: Human cerebellum tissue lysate at 10 µg

  Lane 3: Mouse cerebellum tissue lysate at 10 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 65 kDa

  Observed band size: 45 kDa

  Exposure time: 1min

• 

  Indirect ELISA - Anti-PLBD2 antibody [EPR21204] (ab225580)

  Different concentrations of immobilised PLBD2 protein was recognised in an indirect ELISA by ab225580. Bound ab225580 (1 μg x mL-1) was detected by peroxidase conjugated goat anti-rabbit IgG (0.16 μg x mL-1). OD (background signal subtracted) shown on the y-axis.