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Rabbit Recombinant Monoclonal PLBD2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866), expandable thumbnail
  • Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Putative phospholipase.

Alternative names

Putative phospholipase B-like 2, 76 kDa protein, LAMA-like protein 2, Lamina ancestor homolog 2, Phospholipase B domain-containing protein 2, p76, PLBD2

Recommended products

Rabbit Recombinant Monoclonal PLBD2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21742
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866), expandable thumbnail

    Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 59 seconds

    Lane 3: 15 seconds.

    Lanes 4 & 5: 3 seconds.

    Lanes 6 & 7: 3 minutes.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

    All lanes: Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1: U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

    Lane 2: Human fetal spleen tissue lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lane 6: Human fetal kidney tissue lysate at 20 µg

    Lane 7: Human fetal brain tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa

    Observed band size: 28 kDa, 32 kDa, 76 kDa

  • Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866), expandable thumbnail

    Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 6 seconds.

    Lanes 4-6: 26 seconds.

    Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID:17007843, PMID: 17105447).

    This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

    All lanes: Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Lane 3: Mouse liver tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Lane 5: Rat liver tissue lysate at 10 µg

    Lane 6: Rat spleen tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa

    Observed band size: 28 kDa, 32 kDa, 76 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com