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AB216866

Anti-PLBD2 antibody [EPR21742]

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(1 Publication)

Rabbit Recombinant Monoclonal PLBD2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Putative phospholipase B-like 2, 76 kDa protein, LAMA-like protein 2, Lamina ancestor homolog 2, Phospholipase B domain-containing protein 2, p76, PLBD2

2 Images
Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866)
  • WB

Supplier Data

Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2 : 59 seconds

Lane 3 : 15 seconds.

Lanes 4 & 5 : 3 seconds.

Lanes 6 & 7 : 3 minutes.

Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID : 17007843, PMID : 17105447).

This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

All lanes:

Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Human fetal spleen tissue lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

Lane 6:

Human fetal kidney tissue lysate at 20 µg

Lane 7:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 28 kDa,32 kDa,76 kDa

true

Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866)
  • WB

Supplier Data

Western blot - Anti-PLBD2 antibody [EPR21742] (AB216866)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-3 : 6 seconds.

Lanes 4-6 : 26 seconds.

Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID : 17007843, PMID : 17105447).

This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

All lanes:

Western blot - Anti-PLBD2 antibody [EPR21742] (ab216866) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Lane 3:

Mouse liver tissue lysate at 10 µg

Lane 4:

Rat brain tissue lysate at 10 µg

Lane 5:

Rat liver tissue lysate at 10 µg

Lane 6:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 28 kDa,32 kDa,76 kDa

true

  • Carrier free

    Anti-PLBD2 antibody [EPR21742] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21742

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative phospholipase.
See full target information PLBD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1678176 PubMed41050673

2025

CXCR1 fate mapping distinguishes cochlear resident and recruited macrophages after acoustic trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Sree Varshini Murali,Andrew R Stothert,Elyssa Pereyra,Lyudmila Batalkina,Tejbeer Kaur
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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