Anti-PLBD2 antibody [EPR21742] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (AB231825)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1 & 2 : 59 seconds

Lane 3 : 15 seconds.

Lanes 4 & 5 : 3 seconds.

Lanes 6 & 7 : 3 minutes.

Full-length PLBD2 (76 kDa) can be cleaved into N-terminal and C-terminal fragments according to the literature (PMID : 17007843, PMID : 17105447).

This antibody recognizes both full-length and the N-terminal fragment of PLBD2.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216866).

All lanes:

Western blot - Anti-PLBD2 antibody [EPR21742] - BSA and Azide free (ab231825) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Human fetal spleen tissue lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MEF (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Lane 6:

Human fetalkidney tissue lysate at 20 µg

Lane 7:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 28 kDa,32 kDa,76 kDa

true