Rabbit Polyclonal PLCD1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLCD1 aa 500-750.
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Publications
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- The Journal of investigative dermatology 139:2154-2163.e52019A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteffen Hörer et. al.PubMed 31082376
- PloS one 10:e01237692015Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.Applications:IHC-P, WBReactive species:Unspecified reactive speciesDelphine M Béziau et. al.PubMed 25875657
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLCD1 aa 500-750. The exact immunogen used to generate this antibody is proprietary information. Database link P51178
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (PubMed:9188725). Essential for trophoblast and placental development (By similarity). Binds phosphatidylinositol 4,5-bisphosphate (PubMed:7890667, PubMed:9188725).
Alternative names
Phosphoinositide phospholipase C-delta-1, Phospholipase C-III, Phospholipase C-delta-1, PLC-III, PLC-delta-1, PLCD1
Recommended products
Rabbit Polyclonal PLCD1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLCD1 aa 500-750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLCD1 aa 500-750. The exact immunogen used to generate this antibody is proprietary information. Database link P51178
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-PLCD1 antibody (ab154610)
7.5% SDS PAGE
All lanes: Western blot - Anti-PLCD1 antibody (ab154610) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (ab154610)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (ab154610)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Product protocols
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