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AB154610

Anti-PLCD1 antibody

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(2 Publications)

Rabbit Polyclonal PLCD1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLCD1 aa 500-750.

View Alternative Names

Phosphoinositide phospholipase C-delta-1, Phospholipase C-III, Phospholipase C-delta-1, PLC-III, PLC-delta-1, PLCD1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (AB154610)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PLCD1 antibody (AB154610)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling PLCD1 with ab154610 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (AB154610)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody (AB154610)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PLCD1 with ab154610 at 1/250 dilution.

Western blot - Anti-PLCD1 antibody (AB154610)
  • WB

Unknown

Western blot - Anti-PLCD1 antibody (AB154610)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PLCD1 antibody (ab154610) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PLCD1 aa 500-750. The exact immunogen used to generate this antibody is proprietary information.

P51178

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (PubMed : 9188725). Essential for trophoblast and placental development (By similarity). Binds phosphatidylinositol 4,5-bisphosphate (PubMed : 7890667, PubMed : 9188725).
See full target information PLCD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 139:2154-2163.e5 PubMed31082376

2019

A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Hörer,Slaheddine Marrakchi,Franz P W Radner,Gerd Zolles,Lisa Heinz,Thomas O Eichmann,Cristina Has,Pavel Salavei,Nadia Mahfoudh,Hamida Turki,Andreas D Zimmer,Judith Fischer

PloS one 10:e0123769 PubMed25875657

2015

Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells.

Applications

IHC-P, WB

Species

Unspecified reactive species, Unspecified reactive species

Delphine M Béziau,Fanny Toussaint,Alexandre Blanchette,Nour R Dayeh,Chimène Charbel,Jean-Claude Tardif,Jocelyn Dupuis,Jonathan Ledoux
View all publications
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Product promise

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