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AB134936

Anti-PLCD1 antibody [EPR8430]

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(5 Publications)

Rabbit Recombinant Monoclonal PLCD1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Phosphoinositide phospholipase C-delta-1, Phospholipase C-III, Phospholipase C-delta-1, PLC-III, PLC-delta-1, PLCD1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)

Immunohistochemical analysis of paraffin-embedded formalin-fixed Human testis tissue labelling PLCD1 using ab134936 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)

Immunohistochemical analysis of paraffin-embedded formalin-fixed Human brain tissue labelling PLCD1 using ab134931 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PLCD1 antibody [EPR8430] (AB134936)
  • WB

Unknown

Western blot - Anti-PLCD1 antibody [EPR8430] (AB134936)

All lanes:

Western blot - Anti-PLCD1 antibody [EPR8430] (ab134936) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal lung tissue lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa

true

  • Carrier free

    Anti-PLCD1 antibody [EPR8430] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8430

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (PubMed : 9188725). Essential for trophoblast and placental development (By similarity). Binds phosphatidylinositol 4,5-bisphosphate (PubMed : 7890667, PubMed : 9188725).
See full target information PLCD1

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:4488640 PubMed35836489

2022

PLCD1-Induced DNA Damage Inhibits the Tumor Growth via Downregulating CDKs in Chondrosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiakang Shen,Chen Yu,Zhuoying Wang,Haoran Mu,Zhengdong Cai

Oncology letters 24:252 PubMed35761947

2022

Origin recognition complex 1 regulates phospholipase Cδ1 to inhibit cell proliferation, migration and epithelial-mesenchymal transition in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Jian,Qing Qiao,Juanjuan Tang,Xiaobing Qin

Cell reports 31:107807 PubMed32579933

2020

Identification of Conserved Proteomic Networks in Neurodegenerative Dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Swarup,Timothy S Chang,Duc M Duong,Eric B Dammer,Jingting Dai,James J Lah,Erik C B Johnson,Nicholas T Seyfried,Allan I Levey,Daniel H Geschwind

FASEB bioAdvances 1:465-480 PubMed32123844

2019

IQGAP1 activates PLC-δ1 by direct binding and moving along microtubule with DLC-1 to cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Tanaka,Tomohiro Osanai,Yoshimi Homma,Kenji Hanada,Ken Okumura,Hirofumi Tomita

Oncotarget 8:29056-29066 PubMed28423710

2017

Phospholipase Cδ1 suppresses cell migration and invasion of breast cancer cells by modulating KIF3A-mediated ERK1/2/β- catenin/MMP7 signalling.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Qing Shao,Xinrong Luo,Dejuan Yang,Can Wang,Qiao Cheng,Tingxiu Xiang,Guosheng Ren
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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