Rabbit Recombinant Monoclonal PLCD1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-PLCD1 antibody [EPR8430] (AB134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--western-blot-img122902.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLCD1 antibody [EPR8430] (AB134936)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46510.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135363.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (AB134936)")
Publications
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- Journal of oncology 2022:44886402022PLCD1-Induced DNA Damage Inhibits the Tumor Growth via Downregulating CDKs in Chondrosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiakang Shen,Chen Yu,Zhuoying Wang,Haoran Mu,Zhengdong CaiPubMed 35836489
- Cell reports 31:1078072020Identification of Conserved Proteomic Networks in Neurodegenerative Dementia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVivek Swarup,Timothy S Chang,Duc M Duong,Eric B Dammer,Jingting Dai,James J Lah,Erik C B Johnson,Nicholas T Seyfried,Allan I Levey,Daniel H GeschwindPubMed 32579933
- FASEB bioAdvances 1:465-4802019IQGAP1 activates PLC-δ1 by direct binding and moving along microtubule with DLC-1 to cell surface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Tanaka et. al.PubMed 32123844
- Oncotarget 8:29056-290662017Phospholipase Cδ1 suppresses cell migration and invasion of breast cancer cells by modulating KIF3A-mediated ERK1/2/β- catenin/MMP7 signalling.Applications:IHC, WBReactive species:Unspecified reactive speciesQing Shao,Xinrong Luo,Dejuan Yang,Can Wang,Qiao Cheng,Tingxiu Xiang,Guosheng RenPubMed 28423710
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes (PubMed:9188725). Essential for trophoblast and placental development (By similarity). Binds phosphatidylinositol 4,5-bisphosphate (PubMed:7890667, PubMed:9188725).
Alternative names
Phosphoinositide phospholipase C-delta-1, Phospholipase C-III, Phospholipase C-delta-1, PLC-III, PLC-delta-1, PLCD1
Recommended products
Rabbit Recombinant Monoclonal PLCD1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8430
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PLCD1 antibody [EPR8430] (ab134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--western-blot-img122902.jpg "Western blot - Anti-PLCD1 antibody [EPR8430] (ab134936)")
Western blot - Anti-PLCD1 antibody [EPR8430] (ab134936)
All lanes: Western blot - Anti-PLCD1 antibody [EPR8430] (ab134936) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Human fetal lung tissue lysate at 10 µg
SecondaryAll lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46510.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling PLCD1 using ab134936 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936), expandable thumbnail](https://content.abcam.com/products/images/plcd1-antibody-epr8430-ab134936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135363.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCD1 antibody [EPR8430] (ab134936)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain tissue, labelling PLCD1 using Anti-Mast Cell Tryptase antibody [EPR8475] ab134931 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Product protocols
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- Visit troubleshooting
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