Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]

• WB

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Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

Lane 2:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg

Lane 3:

Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 148 kDa

Observed band size: 150 kDa

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• WB

Unknown

Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)

All lanes:

Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

Lane 1:

Raji cell lysate (untreated) at 10 µg

Lane 2:

Raji cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 148 kDa

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• Dot

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Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)

Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.