Rabbit Monoclonal PLCG 2 phospho Y1217 antibody. Suitable for WB, Dot and reacts with Human samples.
Images
![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--western-blot-img14250.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)")
![Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--dot-blot-img11951.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)")
![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--western-blot-img128063.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Alternative names
Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2
Recommended products
Rabbit Monoclonal PLCG 2 phospho Y1217 antibody. Suitable for WB, Dot and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1404Y
- Purification technique
- Affinity purification Protein A
- Specificity
ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--western-blot-img14250.jpg "Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)")
Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution
Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg
Lane 2: Ramos treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg
Lane 3: Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa
![Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--dot-blot-img11951.jpg "Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)")
Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610), expandable thumbnail](https://content.abcam.com/products/images/plcg-2-phospho-y1217-antibody-ep1404y-ab52610--western-blot-img128063.jpg "Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)")
Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
All lanes: Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution
Lane 1: Raji cell lysate (untreated) at 10 µg
Lane 2: Raji cell lysate treated with pervanadate at 10 µg
SecondaryAll lanes: HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
Downloads
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