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Rabbit Polyclonal PLCG 2 phospho Y753 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PLCG2 phospho Y753.

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Images

Western blot - Anti-PLCG 2 (phospho Y753) antibody (AB75659), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (AB75659), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human PLCG2 phospho Y753. The exact immunogen used to generate this antibody is proprietary information. Database link P16885

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/200

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Alternative names

Recommended products

Rabbit Polyclonal PLCG 2 phospho Y753 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PLCG2 phospho Y753.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human PLCG2 phospho Y753. The exact immunogen used to generate this antibody is proprietary information. Database link P16885
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659), expandable thumbnail

    Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659)

    All lanes: Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution

    Lane 1: A431 cell extracts, untreated

    Lane 2: A431 cell extracts treated with EGF (200ng/ml, 5min)

    Predicted band size: 148 kDa

    Observed band size: 148 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (ab75659), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (ab75659)

    ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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