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AB75659

Anti-PLCG 2 (phospho Y753) antibody

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(2 Publications)

Rabbit Polyclonal PLCG 2 phospho Y753 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLCG2 phospho Y753.

View Alternative Names

Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (AB75659)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (AB75659)

ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.

Western blot - Anti-PLCG 2 (phospho Y753) antibody (AB75659)
  • WB

Unknown

Western blot - Anti-PLCG 2 (phospho Y753) antibody (AB75659)

All lanes:

Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution

Lane 1:

A431 cell extracts, untreated

Lane 2:

A431 cell extracts treated with EGF (200ng/ml, 5min)

Predicted band size: 148 kDa

Observed band size: 148 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PLCG2 phospho Y753. The exact immunogen used to generate this antibody is proprietary information.

P16885

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
See full target information PLCG2 phospho Y753
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 12: PubMed32370190

2020

Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laia Sadeghi,Gustav Arvidsson,Magali Merrien,Agata M Wasik,André Görgens,C I Edvard Smith,Birgitta Sander,Anthony P Wright

Molecular and cellular biology 34:2961-80 PubMed24891615

2014

Chromatinized protein kinase C-θ directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anjum Zafar,Fan Wu,Kristine Hardy,Jasmine Li,Wen Juan Tu,Robert McCuaig,Janelle Harris,Kum Kum Khanna,Joanne Attema,Philip A Gregory,Gregory J Goodall,Kirsti Harrington,Jane E Dahlstrom,Tara Boulding,Rebecca Madden,Abel Tan,Peter J Milburn,Sudha Rao
View all publications
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Product promise

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