AB51020

Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]

Name: Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) | Abcam
Brand: Abcam Limited
SKU: AB51020
Price: 315 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications )

Rabbit Recombinant Monoclonal PLCG 2 phospho Y759 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 2 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (AB51020)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (AB51020)

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Lab

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (AB51020)

All lanes:

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution

Lane 1:

Raji cell lysate, untreated at 10 µg

Lane 2:

Raji cell lysate, treated with pervanadate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 150 kDa

false

Carrier free

Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1406Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/100000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

See full target information PLCG2 pY759

Alternative Names

Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 180:287-307 PubMed36166754

2022

Asebogenin suppresses thrombus formation via inhibition of Syk phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Xulin Xu,Keyu Lv,Guijuan Zheng,Hao Wang,Shuai Chen,Lang Huang,Yi Liu,Yadong Zhang,Zhaoming Tang,Lili Zhang,Jinyu Wang,Jianlin Qiao,Hongliang Li,Xuanbin Wang,Guangmin Yao,Chao Fang

Molecular and cellular biology 33:3214-26 PubMed23754751

2013

Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3ζ, regulates shuttling, and attenuates both tonic and induced signaling in B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dara K Mohammad,Beston F Nore,Alamdar Hussain,Manuela O Gustafsson,Abdalla J Mohamed,C I Edvard Smith

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]

Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (AB51020)

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (AB51020)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (2)

Product promise