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AB277634

Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal PLCG 2 phospho Y759 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, PLCG2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free (AB277634)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free (AB277634)

This data was developed using ab51020, the same antibody clone in a different buffer formulation.

Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free (AB277634)
  • WB

Lab

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] - BSA and Azide free (AB277634)

This data was developed using ab51020, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (<a href='/en-us/products/primary-antibodies/plcg-2-phospho-y759-antibody-ep1406y-ab51020'>ab51020</a>) at 1/100000 dilution

Lane 1:

Raji cell lysate, untreated at 10 µg

Lane 2:

Raji cell lysate, treated with pervanadate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 150 kDa

false

  • Unconjugated

    Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1406Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects PLCG 2 phosphorylated on Tyrosine 759.

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Alternative Products

Primary Antibodies

AB133522

Anti-PLCG 2 antibody [EPR5914-34]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PLCG 2 antibody [EPR5914-34] (AB133522)

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Primary Antibodies

AB75970

Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y]

RabMAb|Trial Size

Western blot - Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (AB75970)

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Primary Antibodies

AB52610

Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]

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Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (AB52610)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab277634 is the carrier-free version of ab51020.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
See full target information PLCG2 phospho Y759
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com