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AB249305

Anti-PLCL1 antibody [EPR11213] - BSA and Azide free

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Rabbit Recombinant Monoclonal PLCL1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Inactive phospholipase C-like protein 1, PLC-L1, Phospholipase C-deleted in lung carcinoma, Phospholipase C-related but catalytically inactive protein, PRIP, PLCL1

4 Images
Flow Cytometry (Intracellular) - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)

This data was developed using ab157200, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

Western blot - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)
  • WB

Unknown

Western blot - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)

This data was developed using ab157200, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLCL1 antibody [EPR11213] (<a href='/en-us/products/primary-antibodies/plcl1-antibody-epr11213-ab157200'>ab157200</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human fetal lung tissue lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Predicted band size: 123 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)

This data was developed using ab157200, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution.

Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)
  • IP

Unknown

Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] - BSA and Azide free (AB249305)

This data was developed using ab157200, the same antibody clone in a different buffer formulation.Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP.

All lanes:

Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (<a href='/en-us/products/primary-antibodies/plcl1-antibody-epr11213-ab157200'>ab157200</a>)

Predicted band size: 123 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11213

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249305 is the carrier-free version of ab157200.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as an inhibitor of PPP1C.
See full target information PLCL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com