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AB115514

Anti-Pleckstrin antibody

3

(1 Review)

|

(2 Publications)

Goat Polyclonal Pleckstrin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PLEK aa 300 to C-terminus.

View Alternative Names

P47, PLEK, Pleckstrin, Platelet 47 kDa protein, p47

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleckstrin antibody (AB115514)

ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-Pleckstrin antibody (AB115514)
  • WB

Unknown

Western blot - Anti-Pleckstrin antibody (AB115514)

Primary incubation : 1 hour

All lanes:

Western blot - Anti-Pleckstrin antibody (ab115514) at 0.05 µg/mL

All lanes:

Human PBMC lysate (in RIPA buffer) at 35 µg

Predicted band size: 40 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PLEK aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08567

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Alternative Products

Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.05-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pleckstrin also known as PLEK is a phosphoprotein with a mass of approximately 40 kDa. It is predominantly expressed in platelets and leukocytes. Pleckstrin is the major protein kinase C substrate in platelets and acts as an important mediator in signal transduction processes. Its structure includes pleckstrin homology domains which play key roles in interacting with other cellular proteins and in lipid binding.
Biological function summary

Pleckstrin participates in cellular processes like cytoskeletal rearrangement and cell motility. It functions as part of protein signaling complexes that regulate actin cytoskeleton dynamics. Through its pleckstrin homology domains pleckstrin binds to phosphatidylinositol lipids which is critical for recruitment to membrane sites required for cellular activation and response.

Pathways

Pleckstrin is intricately involved in the PI3K/AKT signaling pathway and the platelet activation pathway. Within these pathways pleckstrin interacts with proteins like G-protein coupled receptors and PI3K facilitating cellular responses to external stimuli. These processes ensure platelets function correctly during hemostasis and inflammatory responses.

Pleckstrin's role is critical in diseases like thrombocytopenia and psoriasis. Aberrations in pleckstrin expression or function can lead to improper platelet function contributing to abnormal bleeding or clotting scenarios in thrombocytopenia. It is also connected with proteins like PIP3 and AKT which are involved in pathways that can become dysregulated in chronic inflammatory disorders such as psoriasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major protein kinase C substrate of platelets.
See full target information PLEK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 28:76 PubMed35768768

2022

Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yin,Yaofa Lin,Peilin Wang,Jun Zhou,Haodong Lin

Human molecular genetics 29:1520-1536 PubMed32337552

2020

A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Lezirovitz,Gleiciele A Vieira-Silva,Ana C Batissoco,Débora Levy,Joao P Kitajima,Alix Trouillet,Ellen Ouyang,Navid Zebarjadi,Juliana Sampaio-Silva,Vinicius Pedroso-Campos,Larissa R Nascimento,Cindy Y Sonoda,Vinícius M Borges,Laura G Vasconcelos,Roberto M O Beck,Signe S Grasel,Daniel J Jagger,Nicolas Grillet,Ricardo F Bento,Regina C Mingroni-Netto,Jeanne Oiticica
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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