AB134098

Anti-Pleckstrin antibody [EPR6642]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Pleckstrin antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

P47, PLEK, Pleckstrin, Platelet 47 kDa protein, p47

1 Images

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Western blot - Anti-Pleckstrin antibody [EPR6642] (AB134098)

All lanes:

Western blot - Anti-Pleckstrin antibody [EPR6642] (ab134098) at 1/1000 dilution

Lane 1:

Human platelet lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Raji lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

Carrier free

Anti-Pleckstrin antibody [EPR6642] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6642

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pleckstrin also known as PLEK is a phosphoprotein with a mass of approximately 40 kDa. It is predominantly expressed in platelets and leukocytes. Pleckstrin is the major protein kinase C substrate in platelets and acts as an important mediator in signal transduction processes. Its structure includes pleckstrin homology domains which play key roles in interacting with other cellular proteins and in lipid binding.

Biological function summary

Pleckstrin participates in cellular processes like cytoskeletal rearrangement and cell motility. It functions as part of protein signaling complexes that regulate actin cytoskeleton dynamics. Through its pleckstrin homology domains pleckstrin binds to phosphatidylinositol lipids which is critical for recruitment to membrane sites required for cellular activation and response.

Pathways

Pleckstrin is intricately involved in the PI3K/AKT signaling pathway and the platelet activation pathway. Within these pathways pleckstrin interacts with proteins like G-protein coupled receptors and PI3K facilitating cellular responses to external stimuli. These processes ensure platelets function correctly during hemostasis and inflammatory responses.

Pleckstrin's role is critical in diseases like thrombocytopenia and psoriasis. Aberrations in pleckstrin expression or function can lead to improper platelet function contributing to abnormal bleeding or clotting scenarios in thrombocytopenia. It is also connected with proteins like PIP3 and AKT which are involved in pathways that can become dysregulated in chronic inflammatory disorders such as psoriasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major protein kinase C substrate of platelets.

See full target information PLEK

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pleckstrin antibody [EPR6642]

Western blot - Anti-Pleckstrin antibody [EPR6642] (AB134098)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise