AB32528

Anti-Plectin antibody [E398P]

Name: Anti-Plectin antibody [E398P] (ab32528) | Abcam
Brand: Abcam Limited
SKU: AB32528
Price: 240 USD
Availability: InStock
Rating: 5 (6 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

(6 Reviews)

(40 Publications)

Anti-Plectin antibody [E398P] (ab32528) is a rabbit monoclonal antibody detecting Plectin in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2006

View Alternative Names

PLEC1, PLEC, Plectin, PCN, PLTN, Hemidesmosomal protein 1, Plectin-1, HD1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Collaborator

Western blot - Anti-Plectin antibody [E398P] (AB32528)

ab32528 was shown to react with PLEC in wild-type U-2 OS cells in Western blot with loss of signal observed in a PLEC knockout cell line. Wild-type U-2 OS and PLEC knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab32528 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Plectin antibody [E398P] (ab32528) at 1/5000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

PLEC knockout U-2 OS cell lysate at 20 µg

Predicted band size: 532 kDa

false

Carrier free

Anti-Plectin antibody [E398P] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E398P

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises plectin, a 500-kDa intermediate filament binding protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Plectin antibody [E398P] (ab32528) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Plectin?
Anti-Plectin [E398P] (ab32528) specifically detects a band for Plectin (UniProt: Q15149) at a molecular weight of 532kDa.

Trusted by the scientific community
Anti-Plectin [E398P] (ab32528) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Plectin [E398P] (ab32528) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Plectin antibody [E398P] (ab32528) has been confirmed by Western blot testing in PLEC Knockout U2OS cell line.

Other related products
We have a range of other formats of antibody clone [E398P] also available for your convenience: ab32528, Carrier free - ab176475

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plectin also known as PLEC is a large protein with a molecular mass of approximately 500 kDa. It serves as a cross-linking element that plays an important role in maintaining the structural integrity of various cells. Plectin is widely expressed in tissues such as muscle skin and nervous tissue. Its mechanical function involves linking intermediate filaments to membrane complexes and other cytoskeletal structures which supports cellular architecture and stability.

Biological function summary

Plectin contributes significantly to the structural organization of cells and tissues. It is a part of the cytoskeletal complex and assists in cellular integrity by interacting with actin microfilaments microtubules and intermediate filaments. Plectin provides elasticity to cells allowing them to withstand mechanical stress. It ensures proper cellular functions and structural dynamics making it indispensable for maintaining tissue homeostasis.

Pathways

Plectin integrates into cellular signaling networks that control cytoskeletal organization and cell-matrix interactions. It plays a role in the integrin-mediated signaling pathway facilitating communication between the extracellular matrix and the cytoskeleton. Plectin interacts with proteins such as dystrophin which is involved in muscular pathways contributing to cell adhesion and motility.

Mutations or defects in the plectin gene can lead to conditions like epidermolysis bullosa simplex and muscular dystrophies. These disorders illustrate the important role that plectin plays in maintaining structural cell stability. In muscular dystrophies the interaction between plectin and dystrophin gets disrupted affecting muscle fiber integrity and leading to muscle weakness or degeneration. Such associations highlight plectin's significance in pathophysiological contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.

See full target information PLEC

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:15 PubMed39915800

2025

Plectin, a novel regulator in migration, invasion and adhesion of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lanning Bai,Xueqian Qian,Hui Zhang,Yi Yuan,Xiaodong Cui,Min Cheng,Yangyang Han

BMC biology 23:8 PubMed39789506

2025

High matrix stiffness accelerates migration of hepatocellular carcinoma cells through the integrin β1-Plectin-F-actin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Wang,Wenbin Wang,Qing Luo,Guanbin Song

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 55:60-66 PubMed38322534

2024

[Plectin Promotes the Migration of Hepatocellular Carcinoma Cells Through Inducing F-actin Polymerization].

Applications

Unspecified application

Species

Unspecified reactive species

Rushuang Xu,Lingxiao Yang,Guanbin Song

Fluids and barriers of the CNS 20:93 PubMed38098084

2023

Structural characterization of SLYM-a 4th meningeal membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Plá,Styliani Bitsika,Michael J Giannetto,Antonio Ladron-de-Guevara,Daniel Gahn-Martinez,Yuki Mori,Maiken Nedergaard,Kjeld Møllgård

JCI insight 8: PubMed37159335

2023

Restoring calcium homeostasis in Purkinje cells arrests neurodegeneration and neuroinflammation in the ARSACS mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Del Bondio,Fabiana Longo,Daniele De Ritis,Erica Spirito,Paola Podini,Bernard Brais,Angela Bachi,Angelo Quattrini,Francesca Maltecca

International journal of molecular sciences 24: PubMed36613521

2022

Plectin Downregulation Inhibits Migration and Suppresses Epithelial Mesenchymal Transformation of Hepatocellular Carcinoma Cells via ERK1/2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rushuang Xu,Shan He,Di Ma,Rui Liang,Qing Luo,Guanbin Song

Nature communications 13:7529 PubMed36477027

2022

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Applications

Unspecified application

Species

Unspecified reactive species

S Mouron,M J Bueno,A Lluch,L Manso,I Calvo,J Cortes,J A Garcia-Saenz,M Gil-Gil,N Martinez-Janez,J V Apala,E Caleiras,Pilar Ximénez-Embún,J Muñoz,L Gonzalez-Cortijo,R Murillo,R Sánchez-Bayona,J M Cejalvo,G Gómez-López,C Fustero-Torre,S Sabroso-Lasa,N Malats,M Martinez,A Moreno,D Megias,M Malumbres,R Colomer,M Quintela-Fandino

Nature communications 13:3897 PubMed35794106

2022

Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Humberto Mestre,Natasha Verma,Thom D Greene,LiJing A Lin,Antonio Ladron-de-Guevara,Amanda M Sweeney,Guojun Liu,V Kaye Thomas,Chad A Galloway,Karen L de Mesy Bentley,Maiken Nedergaard,Rupal I Mehta

Cells 10: PubMed34571866

2021

A Novel Monoclonal Antibody Targeting Cancer-Specific Plectin Has Potent Antitumor Activity in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha M Perez,Julien Dimastromatteo,Charles N Landen,Kimberly A Kelly

International journal of molecular sciences 22: PubMed33916959

2021

hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination.

Applications

Unspecified application

Species

Unspecified reactive species

Nadezhda A Evtushenko,Arkadii K Beilin,Erdem B Dashinimaev,Rustam H Ziganshin,Anastasiya V Kosykh,Maxim M Perfilov,Alexandra L Rippa,Elena V Alpeeva,Andrey V Vasiliev,Ekaterina A Vorotelyak,Nadya G Gurskaya

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plectin antibody [E398P]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

Western blot - Anti-Plectin antibody [E398P] (AB32528)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (40)

Product promise