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AB176475

Anti-Plectin antibody [E398P] - BSA and Azide free

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(1 Publication)

Anti-Plectin antibody [E398P] - BSA and Azide free (ab176475) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Plectin in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

PLEC1, PLEC, Plectin, PCN, PLTN, Hemidesmosomal protein 1, Plectin-1, HD1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)

Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32528).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)

Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32528).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)
  • WB

Collaborator

Western blot - Anti-Plectin antibody [E398P] - BSA and Azide free (AB176475)

This data was developed using the same antibody in a different buffer formulation (ab32528).

ab32528 was shown to react with PLEC in wild-type U-2 OS cells in Western blot with loss of signal observed in a PLEC knockout cell line. Wild-type U-2 OS and PLEC knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab32528 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Plectin antibody [E398P] (<a href='/en-us/products/primary-antibodies/plectin-antibody-e398p-ab32528'>ab32528</a>) at 1/5000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

PLEC knockout U-2 OS cell lysate at 20 µg

Predicted band size: 532 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E398P

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises plectin, a 500-kDa intermediate filament binding protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Plectin antibody [E398P] - BSA and Azide free (ab176475) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Plectin?
Anti-Plectin [E398P] - BSA and Azide free (ab176475) specifically detects a band for Plectin (UniProt: Q15149) at a molecular weight of 532kDa.

Specificity confirmed
The specificity of Anti-Plectin antibody [E398P] - BSA and Azide free (ab176475) has been confirmed by Western blot testing in PLEC Knockout U2OS cells.

Other related products
We have a range of other formats of antibody clone [E398P] also available for your convenience: ab32528, Carrier free - ab176475

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plectin also known as PLEC is a large protein with a molecular mass of approximately 500 kDa. It serves as a cross-linking element that plays an important role in maintaining the structural integrity of various cells. Plectin is widely expressed in tissues such as muscle skin and nervous tissue. Its mechanical function involves linking intermediate filaments to membrane complexes and other cytoskeletal structures which supports cellular architecture and stability.
Biological function summary

Plectin contributes significantly to the structural organization of cells and tissues. It is a part of the cytoskeletal complex and assists in cellular integrity by interacting with actin microfilaments microtubules and intermediate filaments. Plectin provides elasticity to cells allowing them to withstand mechanical stress. It ensures proper cellular functions and structural dynamics making it indispensable for maintaining tissue homeostasis.

Pathways

Plectin integrates into cellular signaling networks that control cytoskeletal organization and cell-matrix interactions. It plays a role in the integrin-mediated signaling pathway facilitating communication between the extracellular matrix and the cytoskeleton. Plectin interacts with proteins such as dystrophin which is involved in muscular pathways contributing to cell adhesion and motility.

Mutations or defects in the plectin gene can lead to conditions like epidermolysis bullosa simplex and muscular dystrophies. These disorders illustrate the important role that plectin plays in maintaining structural cell stability. In muscular dystrophies the interaction between plectin and dystrophin gets disrupted affecting muscle fiber integrity and leading to muscle weakness or degeneration. Such associations highlight plectin's significance in pathophysiological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
See full target information PLEC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:5565-5583 PubMed37140030

2023

Proper RPA acetylation promotes accurate DNA replication and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Gan,Yueyue Zhang,Donghao Jiang,Jingyao Shi,Han Zhao,Chengyu Xie,Yanyan Wang,Jingyan Xu,Xinghua Zhang,Gang Cai,Hailong Wang,Jun Huang,Xuefeng Chen
View all publications
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