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AB313723

Anti-Plectin antibody [EPR26993-15] - BSA and Azide free (Detector)

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Anti-Plectin antibody [EPR26993-15] - BSA and Azide free (Detector) (ab313723) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Plectin in ELISA. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
1 Images
Sandwich ELISA - Anti-Plectin antibody [EPR26993-15] - BSA and Azide free (Detector) (AB313723)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Plectin antibody [EPR26993-15] - BSA and Azide free (Detector) (AB313723)

Sandwich ELISA of ab313721 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26993-15

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Anti-Plectin antibody [EPR26993-15] - BSA and Azide free (Detector) (ab313723) is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Plectin.
The recommended pair for sandwich ELISA is:
Capture: ab313722, Plectin Capture Antibody (unconjugated)
Detector: ab313723, Plectin Detector Antibody (unconjugated)
The reference range value is 78.1-5000 pg/ml.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plectin also known as PLEC is a large protein with a molecular mass of approximately 500 kDa. It serves as a cross-linking element that plays an important role in maintaining the structural integrity of various cells. Plectin is widely expressed in tissues such as muscle skin and nervous tissue. Its mechanical function involves linking intermediate filaments to membrane complexes and other cytoskeletal structures which supports cellular architecture and stability.
Biological function summary

Plectin contributes significantly to the structural organization of cells and tissues. It is a part of the cytoskeletal complex and assists in cellular integrity by interacting with actin microfilaments microtubules and intermediate filaments. Plectin provides elasticity to cells allowing them to withstand mechanical stress. It ensures proper cellular functions and structural dynamics making it indispensable for maintaining tissue homeostasis.

Pathways

Plectin integrates into cellular signaling networks that control cytoskeletal organization and cell-matrix interactions. It plays a role in the integrin-mediated signaling pathway facilitating communication between the extracellular matrix and the cytoskeleton. Plectin interacts with proteins such as dystrophin which is involved in muscular pathways contributing to cell adhesion and motility.

Mutations or defects in the plectin gene can lead to conditions like epidermolysis bullosa simplex and muscular dystrophies. These disorders illustrate the important role that plectin plays in maintaining structural cell stability. In muscular dystrophies the interaction between plectin and dystrophin gets disrupted affecting muscle fiber integrity and leading to muscle weakness or degeneration. Such associations highlight plectin's significance in pathophysiological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
See full target information PLEC

Alternative Names

PLEC1, PLEC, Plectin, PCN, PLTN, Hemidesmosomal protein 1, Plectin-1, HD1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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