Anti-Plexin A1 antibody [EPR29121-94] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Plexin A1 antibody [EPR29121-94] - BSA and Azide free (AB321806)

This data was developed using ab321805, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : P815.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16715077, PMID : 12559962, PMID : 25485759, PMID : 35508542).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Plexin A1 antibody [EPR29121-94] (<a href='/en-us/products/primary-antibodies/plexin-a1-antibody-epr29121-94-ab321805'>ab321805</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 50 µg

Lane 3:

P815 (mouse mastocytoma mast cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Plexin A1 antibody [EPR29121-94] - BSA and Azide free (AB321806)

This data was developed using ab321805, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 8806667, PMID : 12559962).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16715077, PMID : 12559962, PMID : 25485759, PMID : 35508542).

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the lower MW band at approximately 37 kDa (in lane 2) is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Plexin A1 antibody [EPR29121-94] (<a href='/en-us/products/primary-antibodies/plexin-a1-antibody-epr29121-94-ab321805'>ab321805</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Plexin A1 antibody [EPR29121-94] - BSA and Azide free (AB321806)

This data was developed using ab321805, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse PLXNA2, PLXNA3 and PLXNA4.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Plexin A1 antibody [EPR29121-94] (<a href='/en-us/products/primary-antibodies/plexin-a1-antibody-epr29121-94-ab321805'>ab321805</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse PLXNA1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse PLXNA2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse PLXNA3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse PLXNA4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

false

Exposure time: 180s