AB92346

Anti-Plexin A1 antibody [EPR3449]

Name: Anti-Plexin A1 antibody [EPR3449] (ab92346) | Abcam
Brand: Abcam Limited
SKU: AB92346
Price: 335 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal Plexin A1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NOV, PLXN1, PLXNA1, Plexin-A1, Semaphorin receptor NOV

2 Images

Flow Cytometry (Intracellular) - Anti-Plexin A1 antibody [EPR3449] (AB92346)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Plexin A1 antibody [EPR3449] (AB92346)

Intracellular Flow Cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).

Unknown

Western blot - Anti-Plexin A1 antibody [EPR3449] (AB92346)

All lanes:

Western blot - Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 211 kDa

false

Carrier free

Anti-Plexin A1 antibody [EPR3449] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3449

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This antibody is not purified. It is provided in cell supernatant and storage buffer.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin A1 also known as PLXNA1 is a transmembrane protein that acts as a receptor for semaphorin proteins. It is involved in signaling pathways that guide cell migration and axonal growth. This protein has a molecular mass of around 220 kDa. Plexin A1 is expressed in several tissues including the nervous system where it plays a significant role in neural development. The expression levels can vary depending on the tissue type indicating its diverse functions across different biological contexts.

Biological function summary

Plexin A1 functions in neural patterning and the immune response. It is a part of semaphorin signaling complexes that include neuropilins and other plexins. These complexes are critical in translating extracellular cues into intracellular responses impacting cellular dynamics such as motility and adhesion. Plexin A1 also interacts with various intracellular signaling molecules influencing actin cytoskeleton reorganization which is essential for proper neural development.

Pathways

Plexin A1 participates in semaphorin signaling and cytoskeletal dynamics pathways. It interacts with Sema3A and Sema4D proteins which are key signaling semaphorins. These interactions regulate cellular movement by influencing the activity of downstream effectors like Rho and Rac GTPases. The involvement of Plexin A1 in these pathways is important for processes like axon guidance and neuronal connectivity.

Plexin A1 has association with cancer progression and neurodevelopmental disorders. Altered expression levels of Plexin A1 can lead to dysfunction in semaphorin-mediated signaling contributing to conditions such as autism spectrum disorders and tumor metastasis. The protein interacts with Met a receptor tyrosine kinase known to be involved in cancer linking Plexin A1 with oncogenic pathways and providing insights into its role in pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Acts as coreceptor of TREM2 for SEMA6D in dendritic cells and is involved in the generation of immune responses and skeletal homeostasis.

See full target information PLXNA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70012 PubMed39508411

2024

PlexinA1 (PLXNA1) as a novel scaffold protein for the engineering of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhao,Zhi Li,Da Liu,Jiaxun Zhang,Zhicheng You,Yuzhang Shao,Hongyan Li,Jun Yang,Xiang Liu,Miaozhu Wang,Chengen Wu,Jing Chen,Jianwu Wang,Guanyi Kong,Libo Zhao

EBioMedicine 39:95-108 PubMed30579864

2018

Semaphorin-3A protects against neointimal hyperplasia after vascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Hong Wu,Yi-Fan Zhou,Can-Dong Hong,An-Qi Chen,Yan Luo,Ling Mao,Yuan-Peng Xia,Quan-Wei He,Hui-Juan Jin,Ming Huang,Ya-Nan Li,Bo Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plexin A1 antibody [EPR3449]

Flow Cytometry (Intracellular) - Anti-Plexin A1 antibody [EPR3449] (AB92346)

Western blot - Anti-Plexin A1 antibody [EPR3449] (AB92346)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise