Rabbit Polyclonal Plexin B1 antibody. Suitable for IHC-P, WB and reacts with Human samples.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496).
Alternative names
KIAA0407, PLXN5, SEP, PLXNB1, Plexin-B1, Semaphorin receptor SEP
Recommended products
Rabbit Polyclonal Plexin B1 antibody. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plexin-B1 also known as PLXNB1 is a transmembrane receptor with a molecular weight of approximately 230 kDa. It plays an important role in cellular communication by binding semaphorin ligands influencing cell adhesion and migration. Plexin-B1 is widely expressed in various tissues including the nervous system where it impacts neural development. By mediating axonal guidance and cytoskeletal dynamics Plexin-B1 contributes to the signaling processes fundamental to neuronal pathfinding and connection establishment.
- Biological function summary
Plexin-B1 associates with multiple proteins to form signaling complexes that regulate cellular processes. Its interaction with small GTPases like RhoA influences actin cytoskeleton organization impacting cell morphology and motility. Plexin-B1 also plays a role in epithelial cell function and survival acting through pathways that restrict or allow cellular proliferation. These interactions facilitate diverse physiological responses including changes in cell shape cellular migration and apoptosis.
- Pathways
Plexin-B1 is involved in signaling cascades such as the semaphorin-plexin pathway and the RhoA/Rho kinase (ROCK) pathway. Through these pathways Plexin-B1 interacts with the receptor tyrosine kinase MET influencing cell motility and invasive growth responses. Plexin-B1 links to Rho GTPases modulating actin dynamics and contributing to cell shape alterations and motility in these pathways. These interactions exemplify Plexin-B1's integration within complex signaling networks that control developmental and cellular processes.
- Associated diseases and disorders
Plexin-B1 shows associations with cancer progression and neural abnormalities. Altered Plexin-B1 expression or function relates to prostate cancer where it participates with MET in promoting epithelial-mesenchymal transition and tumor metastasis. In the neurological domain Plexin-B1 dysfunction connects with schizophrenia by potentially affecting neural connectivity and synaptic integrity. These insights underline Plexin-B1's involvement with critical proteins in pathological contexts influencing the progression and severity of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B1 antibody (ab136880)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labelling Plexin B1 with ab136880 at 5µg/ml.
Western blot - Anti-Plexin B1 antibody (ab136880)
The higher band represents Plexin B1 precursor and the lower band Plexin B1 cleavage product.
All lanes: Western blot - Anti-Plexin B1 antibody (ab136880) at 1/300 dilution
All lanes: HeLa cell membrane extract
Predicted band size: 232 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com