AB281110

Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Plexin B1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

KIAA0407, PLXN5, SEP, PLXNB1, Plexin-B1, Semaphorin receptor SEP

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Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector) (AB281110)

sELISA

Lab

Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector) (AB281110)

Sandwich ELISA of ab253700 with the capture antibody ab281260 dilution at 2 µg/mL and detector antibody ab281110 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23781-169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab281110 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PLXNB1. The recommended pair for sandwich ELISA is:
Capture: ab281260, Human PLXNB1 Capture Antibody (unconjugated)
Detector: ab281110, Human PLXNB1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin-B1 also known as PLXNB1 is a transmembrane receptor with a molecular weight of approximately 230 kDa. It plays an important role in cellular communication by binding semaphorin ligands influencing cell adhesion and migration. Plexin-B1 is widely expressed in various tissues including the nervous system where it impacts neural development. By mediating axonal guidance and cytoskeletal dynamics Plexin-B1 contributes to the signaling processes fundamental to neuronal pathfinding and connection establishment.

Biological function summary

Plexin-B1 associates with multiple proteins to form signaling complexes that regulate cellular processes. Its interaction with small GTPases like RhoA influences actin cytoskeleton organization impacting cell morphology and motility. Plexin-B1 also plays a role in epithelial cell function and survival acting through pathways that restrict or allow cellular proliferation. These interactions facilitate diverse physiological responses including changes in cell shape cellular migration and apoptosis.

Pathways

Plexin-B1 is involved in signaling cascades such as the semaphorin-plexin pathway and the RhoA/Rho kinase (ROCK) pathway. Through these pathways Plexin-B1 interacts with the receptor tyrosine kinase MET influencing cell motility and invasive growth responses. Plexin-B1 links to Rho GTPases modulating actin dynamics and contributing to cell shape alterations and motility in these pathways. These interactions exemplify Plexin-B1's integration within complex signaling networks that control developmental and cellular processes.

Plexin-B1 shows associations with cancer progression and neural abnormalities. Altered Plexin-B1 expression or function relates to prostate cancer where it participates with MET in promoting epithelial-mesenchymal transition and tumor metastasis. In the neurological domain Plexin-B1 dysfunction connects with schizophrenia by potentially affecting neural connectivity and synaptic integrity. These insights underline Plexin-B1's involvement with critical proteins in pathological contexts influencing the progression and severity of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for SEMA4D (PubMed : 19843518, PubMed : 20877282, PubMed : 21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed : 12196628, PubMed : 15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed : 12198496).

See full target information PLXNB1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector) (AB281110)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise