Rabbit Recombinant Monoclonal Plexin B1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-82] - BSA and Azide free (Capture) (AB281260), expandable thumbnail](https://content.abcam.com/products/images/plexin-b1-antibody-epr23781-82-bsa-and-azide-free-capture-ab281260--sandwich-elisa-img438606.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-82] - BSA and Azide free (Capture) (AB281260)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496).
Alternative names
KIAA0407, PLXN5, SEP, PLXNB1, Plexin-B1, Semaphorin receptor SEP
Recommended products
Rabbit Recombinant Monoclonal Plexin B1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23781-82
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab281260 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PLXNB1. The recommended pair for sandwich ELISA is:
Capture: ab281260, Human PLXNB1 Capture Antibody (unconjugated)
Detector: Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector) ab281110, Human PLXNB1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plexin-B1 also known as PLXNB1 is a transmembrane receptor with a molecular weight of approximately 230 kDa. It plays an important role in cellular communication by binding semaphorin ligands influencing cell adhesion and migration. Plexin-B1 is widely expressed in various tissues including the nervous system where it impacts neural development. By mediating axonal guidance and cytoskeletal dynamics Plexin-B1 contributes to the signaling processes fundamental to neuronal pathfinding and connection establishment.
- Biological function summary
Plexin-B1 associates with multiple proteins to form signaling complexes that regulate cellular processes. Its interaction with small GTPases like RhoA influences actin cytoskeleton organization impacting cell morphology and motility. Plexin-B1 also plays a role in epithelial cell function and survival acting through pathways that restrict or allow cellular proliferation. These interactions facilitate diverse physiological responses including changes in cell shape cellular migration and apoptosis.
- Pathways
Plexin-B1 is involved in signaling cascades such as the semaphorin-plexin pathway and the RhoA/Rho kinase (ROCK) pathway. Through these pathways Plexin-B1 interacts with the receptor tyrosine kinase MET influencing cell motility and invasive growth responses. Plexin-B1 links to Rho GTPases modulating actin dynamics and contributing to cell shape alterations and motility in these pathways. These interactions exemplify Plexin-B1's integration within complex signaling networks that control developmental and cellular processes.
- Associated diseases and disorders
Plexin-B1 shows associations with cancer progression and neural abnormalities. Altered Plexin-B1 expression or function relates to prostate cancer where it participates with MET in promoting epithelial-mesenchymal transition and tumor metastasis. In the neurological domain Plexin-B1 dysfunction connects with schizophrenia by potentially affecting neural connectivity and synaptic integrity. These insights underline Plexin-B1's involvement with critical proteins in pathological contexts influencing the progression and severity of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-82] - BSA and Azide free (Capture) (ab281260), expandable thumbnail](https://content.abcam.com/products/images/plexin-b1-antibody-epr23781-82-bsa-and-azide-free-capture-ab281260--sandwich-elisa-img438606.jpg "Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-82] - BSA and Azide free (Capture) (ab281260)")
Sandwich ELISA - Anti-Plexin B1 antibody [EPR23781-82] - BSA and Azide free (Capture) (ab281260)
Sandwich ELISA of Human Plexin B1 Antibody Pair - BSA and Azide free ab253700 with the capture antibody ab281260 dilution at 2 µg/mL and detector antibody Anti-Plexin B1 antibody [EPR23781-169] - BSA and Azide free (Detector) ab281110 dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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