Anti-Plexin B1 antibody [EPR26309-65]

• WB

Supplier Data

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (AB316312)

Low expression : 293T (PMID : 12533544)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (ab316312) at 1/1000 dilution

Lane 1:

HepG2(human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150-300 kDa,36 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (AB316312)

The identity of the bands beneath 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (ab316312) at 1/1000 dilution

Lane 1:

SK-OV-3 (human ovarian cancer epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

SK-OV-3 transfected with siRNA specifically targeting Plexin B1 whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150-300 kDa,36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (AB316312)

Low expression : skeletal muscle(PMID : 12559962)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (ab316312) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150-300 kDa,36 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (AB316312)

This antibody does not cross-react with human PLXNB2 and human PLXNB3.

His tag not visible in empty vector control image as out of range due to small size

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Plexin B1 antibody [EPR26309-65] (ab316312) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T cells transfected with a PLXNB1 expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

293T cells transfected with a PLXNB2 expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 4:

293T cells transfected with a PLXNB3 expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-300 kDa,124 kDa

false

Exposure time: 70s