AB229950

Anti-Plexin B2/MM1 antibody

Name: Anti-Plexin B2/MM1 antibody (ab229950) | Abcam
Brand: Abcam Limited
SKU: AB229950

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Plexin B2/MM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLXNB2 aa 1050-1200.

View Alternative Names

KIAA0315, PLXNB2, Plexin-B2, MM1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B2/MM1 antibody (AB229950)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B2/MM1 antibody (AB229950)

Paraffin-embedded human colon cancer tissue stained for Plexin B2/MM1 using ab229950 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B2/MM1 antibody (AB229950)

Paraffin-embedded human kidney tissue stained for Plexin B2/MM1 using ab229950 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody (AB229950)

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody (ab229950) at 1/1000 dilution

All lanes:

Mouse liver lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 204 kDa

Observed band size: 205 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PLXNB2 aa 1050-1200. The exact immunogen used to generate this antibody is proprietary information.

O15031

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin B2 also known as PLXNB2 or MM1 is a high-molecular-weight protein approximately 220 kDa found in humans. Plexin B2 is involved in the semaphorin receptor complex and is made of multiple domains that interact with semaphorin molecules to mediate cellular functions. Plexin B2 is expressed in various tissues including the brain spinal cord and bones indicating its role in different neural and structural processes. This protein interacts with other members of the Plexin family and undergoes changes that allow it to serve numerous signaling roles in the body.

Biological function summary

Plexin B2 plays a role in cellular signaling processes that are essential for neural patterning and tissue morphogenesis. It binds with semaphorins particularly Sema4C to guide axonal repulsion in neural development. Plexin B2 forms part of a receptor complex that helps cells interpret external semaphorin signals influencing cell movement and positioning. These interactions contribute to the remodeling of neural circuits which is important during both prenatal development and adult neural function.

Pathways

Plexin B2 participates in pathways engaging Rho GTPases which are important for cytoskeletal organization and cell movement. Plexin B2 influences the RhoA and Rac1 pathways impacting actin cytoskeleton dynamics and cell adhesion processes. These pathways where Plexin B2 acts with other Rho family proteins help transmit the changes induced by semaphorin binding altering cellular orientation and migration.

Plexin B2 has connections to neural tube defects and certain cancers. Defective Plexin B2 signaling can lead to improper neural tube closure contributing to conditions like spina bifida. Additionally in oncology the dysregulation of Plexin B2 expression or function links to tumor invasiveness and metastasis where it might interact with proteins like MET and Rho GTPases to influence cancer cell behavior. Understanding its role in these disorders aids in developing therapeutic strategies targeting aberrant Plexin B2 signaling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (By similarity). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (By similarity). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (By similarity). Also acts as a cell surface receptor for angiogenin (ANG); promoting ANG endocytosis and translocation to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (By similarity). Regulates the migration of cerebellar granule cells in the developing brain (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed : 12183458). Plays a role in axon guidance, invasive growth and cell migration (PubMed : 15184888). May modulate the activity of RAC1 and CDC42 (By similarity).

See full target information PLXNB2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2206852 PubMed37083227

2023

Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yidong Chen,Xixi Liu,Li Zhang,Feiyin Zhu,Liying Yan,Wenhao Tang,Zhe Zhang,Qiang Liu,Hui Jiang,Jie Qiao

Frontiers in genetics 12:644451 PubMed34367233

2021

Circular RNA Circ_0013958 Functions as a Tumor Promoter in Ovarian Cancer by Regulating miR-637/PLXNB2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfei Liang,Kaiyi Meng,Rui Qiu

Journal of translational medicine 19:123 PubMed33757550

2021

A circular RNA derived from PLXNB2 as a valuable predictor of the prognosis of patients with acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Lin,Yu Wang,Sicheng Bian,Lili Sun,Zhibo Guo,Desheng Kong,Linlin Zhao,Dan Guo,Qi Li,Min Wu,Yuhuang Wang,Yuying Wang,Yinghua Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plexin B2/MM1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B2/MM1 antibody (AB229950)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plexin B2/MM1 antibody (AB229950)

Western blot - Anti-Plexin B2/MM1 antibody (AB229950)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise