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Rabbit Recombinant Monoclonal Plexin B2/MM1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (AB320873), expandable thumbnail
  • Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (AB320873), expandable thumbnail
  • Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (AB320873), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (By similarity). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (By similarity). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (By similarity). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (By similarity). Regulates the migration of cerebellar granule cells in the developing brain (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12183458). Plays a role in axon guidance, invasive growth and cell migration (PubMed:15184888). May modulate the activity of RAC1 and CDC42 (By similarity).

Alternative names

Plexin-B2, MM1, PLXNB2, KIAA0315

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Rabbit Recombinant Monoclonal Plexin B2/MM1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Plexin-B2, MM1, PLXNB2, KIAA0315

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR29380-522

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with human Plexin B3.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab320873 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Plexin B2/MM1. The recommended pair for sandwich ELISA is:

Capture: ab320873, Human Plexin B2/MM1 Capture Antibody (unconjugated)

Detector: Anti-Plexin B2/MM1 antibody [EPR29380-545] - BSA and Azide free (Capture/Detector) ab320874, Human Plexin B2/MM1 Detector Antibody (unconjugated)

The reference range value is 39.06-5000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

Activity summary

Plexin B2 also known as PLXNB2 or MM1 is a high-molecular-weight protein approximately 220 kDa found in humans. Plexin B2 is involved in the semaphorin receptor complex and is made of multiple domains that interact with semaphorin molecules to mediate cellular functions. Plexin B2 is expressed in various tissues including the brain spinal cord and bones indicating its role in different neural and structural processes. This protein interacts with other members of the Plexin family and undergoes changes that allow it to serve numerous signaling roles in the body.

Biological function summary

Plexin B2 plays a role in cellular signaling processes that are essential for neural patterning and tissue morphogenesis. It binds with semaphorins particularly Sema4C to guide axonal repulsion in neural development. Plexin B2 forms part of a receptor complex that helps cells interpret external semaphorin signals influencing cell movement and positioning. These interactions contribute to the remodeling of neural circuits which is important during both prenatal development and adult neural function.

Pathways

Plexin B2 participates in pathways engaging Rho GTPases which are important for cytoskeletal organization and cell movement. Plexin B2 influences the RhoA and Rac1 pathways impacting actin cytoskeleton dynamics and cell adhesion processes. These pathways where Plexin B2 acts with other Rho family proteins help transmit the changes induced by semaphorin binding altering cellular orientation and migration.

Associated diseases and disorders

Plexin B2 has connections to neural tube defects and certain cancers. Defective Plexin B2 signaling can lead to improper neural tube closure contributing to conditions like spina bifida. Additionally in oncology the dysregulation of Plexin B2 expression or function links to tumor invasiveness and metastasis where it might interact with proteins like MET and Rho GTPases to influence cancer cell behavior. Understanding its role in these disorders aids in developing therapeutic strategies targeting aberrant Plexin B2 signaling.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873), expandable thumbnail

    Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873)

    Interpolated concentration of native Plexin B2/MM1 was measured at different sample concentrations. Undiluted samples are as follows: human plasma (EDTA) 2.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873), expandable thumbnail

    Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873)

    Interpolated concentration of native Plexin B2/MM1 was measured at different sample concentrations. Undiluted sample is as follows: HepG2 cell extract (6.25 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873), expandable thumbnail

    Sandwich ELISA - Anti-Plexin B2/MM1 antibody [EPR29380-522] - BSA and Azide free (Capture) (ab320873)

    Sandwich ELISA of Human Plexin B2/MM1 Antibody Pair - BSA and Azide free ab320872 with the capture antibody ab320873 dilution at 2 ug/mL and detector antibody Anti-Plexin B2/MM1 antibody [EPR29380-545] - BSA and Azide free (Capture/Detector) ab320874 dilution at 0.5 ug/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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