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AB193355

Anti-Plexin B2/MM1 antibody [EPR9965]

3

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Plexin B2/MM1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 5 publications.

View Alternative Names

KIAA0315, PLXNB2, Plexin-B2, MM1

5 Images
Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)
  • WB

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

Lanes 1 - 4 : Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.

ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

PLXNB2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-plxnb2-plexin-b2-mm1-knockout-hek-293-cell-line-ab269468'>ab269468</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

Human brain whole cell lysate at 20 µg

Predicted band size: 204 kDa

Observed band size: 80 kDa

false

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)
  • WB

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 204 kDa

false

Flow Cytometry (Intracellular) - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

Intracellular Flow Cytometry analysis of U87-MG cells labeling Plexin B2/MM1 using ab193355 at a 1/90 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control/ colour cells without incubation with primary antibody/ secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)
  • WB

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Raw264.7 lysate at 10 µg

Lane 3:

NIH/3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 204 kDa

false

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)
  • WB

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

Plexin B2/MM1 could be cleaved into two subunits. The band observed at 80 kDa should be a β-chain.

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 204 kDa

false

  • Carrier free

    Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9965

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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You may be interested in:

AB313925

Human Plexin B2/MM1 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin B2 also known as PLXNB2 or MM1 is a high-molecular-weight protein approximately 220 kDa found in humans. Plexin B2 is involved in the semaphorin receptor complex and is made of multiple domains that interact with semaphorin molecules to mediate cellular functions. Plexin B2 is expressed in various tissues including the brain spinal cord and bones indicating its role in different neural and structural processes. This protein interacts with other members of the Plexin family and undergoes changes that allow it to serve numerous signaling roles in the body.
Biological function summary

Plexin B2 plays a role in cellular signaling processes that are essential for neural patterning and tissue morphogenesis. It binds with semaphorins particularly Sema4C to guide axonal repulsion in neural development. Plexin B2 forms part of a receptor complex that helps cells interpret external semaphorin signals influencing cell movement and positioning. These interactions contribute to the remodeling of neural circuits which is important during both prenatal development and adult neural function.

Pathways

Plexin B2 participates in pathways engaging Rho GTPases which are important for cytoskeletal organization and cell movement. Plexin B2 influences the RhoA and Rac1 pathways impacting actin cytoskeleton dynamics and cell adhesion processes. These pathways where Plexin B2 acts with other Rho family proteins help transmit the changes induced by semaphorin binding altering cellular orientation and migration.

Plexin B2 has connections to neural tube defects and certain cancers. Defective Plexin B2 signaling can lead to improper neural tube closure contributing to conditions like spina bifida. Additionally in oncology the dysregulation of Plexin B2 expression or function links to tumor invasiveness and metastasis where it might interact with proteins like MET and Rho GTPases to influence cancer cell behavior. Understanding its role in these disorders aids in developing therapeutic strategies targeting aberrant Plexin B2 signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (By similarity). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (By similarity). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (By similarity). Also acts as a cell surface receptor for angiogenin (ANG); promoting ANG endocytosis and translocation to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (By similarity). Regulates the migration of cerebellar granule cells in the developing brain (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed : 12183458). Plays a role in axon guidance, invasive growth and cell migration (PubMed : 15184888). May modulate the activity of RAC1 and CDC42 (By similarity).
See full target information PLXNB2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e16104 PubMed36722641

2023

Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Brundu,Virginia Napolitano,Giulia Franzolin,Ettore Lo Cascio,Roberta Mastrantonio,Gabriele Sardo,Eliano Cascardi,Federica Verginelli,Sergio Sarnataro,Gennaro Gambardella,Alberto Pisacane,Alessandro Arcovito,Carla Boccaccio,Paolo M Comoglio,Enrico Giraudo,Luca Tamagnone

Nature communications 12:6019 PubMed34650052

2021

Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Chrystian Junqueira Alves,Rafael Dariolli,Jonathan Haydak,Sangjo Kang,Theodore Hannah,Robert J Wiener,Stefanie DeFronzo,Rut Tejero,Gabriele L Gusella,Aarthi Ramakrishnan,Rodrigo Alves Dias,Alexandre Wojcinski,Santosh Kesari,Li Shen,Eric A Sobie,José Paulo Rodrigues Furtado de Mendonça,Evren U Azeloglu,Hongyan Zou,Roland H Friedel

Cell death discovery 7:164 PubMed34226511

2021

The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.

Applications

Unspecified application

Species

Unspecified reactive species

María Del Carmen Conde-Rubio,Roman Mylonas,Christian Widmann

Communications biology 4:145 PubMed33514835

2021

Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanics.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Huang,Rut Tejero,Vivian K Lee,Concetta Brusco,Theodore Hannah,Taylor B Bertucci,Chrystian Junqueira Alves,Igor Katsyv,Michael Kluge,Ramsey Foty,Bin Zhang,Caroline C Friedel,Guohao Dai,Hongyan Zou,Roland H Friedel

The EMBO journal 39:e103325 PubMed32510170

2020

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rongpan Bai,Desen Sun,Muxiong Chen,Xiaoliang Shi,Liang Luo,Zhengrong Yao,Yaxin Liu,Xiaolong Ge,Xiangwei Gao,Guo-Fu Hu,Wei Zhou,Jinghao Sheng,Zhengping Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB229950

Anti-Plexin B2/MM1 antibody

primary-antibodies

plexin-b2-mm1-antibody-ab229950

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(0 reviews)

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