Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (AB251169)

Lanes 1 - 4 : Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.

ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193355).

All lanes:

Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (<a href='/en-us/products/primary-antibodies/plexin-b2-mm1-antibody-epr9965-ab193355'>ab193355</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

PLXNB2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-plxnb2-plexin-b2-mm1-knockout-hek-293-cell-line-ab269468'>ab269468</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

Human brain whole cell lysate at 20 µg

Predicted band size: 204 kDa

Observed band size: 80 kDa

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