AB28762

Anti-Plexin D1 antibody

Name: Anti-Plexin D1 antibody. Goat polyclonal (ab28762) | Abcam
Brand: Abcam Limited
SKU: AB28762
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(15 Publications)

Goat Polyclonal Plexin D1 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human PLXND1 aa 1650-1700.

View Alternative Names

KIAA0620, PLXND1, Plexin-D1

3 Images

Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (AB28762)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (AB28762)

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Incubated with ab28762 for 1 hour (10 μg/ml) followed by Alexa Fluor^® 488 secondary antibody (2 μg/ml).

IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB28762)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB28762)

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB28762)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human PLXND1 aa 1650-1700. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4D7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin D1 also known as PLXND1 is a transmembrane receptor that plays an important role in cell signaling. It belongs to the plexin family and has a molecular weight of roughly 250 kDa. The plexin D1 receptor is widely expressed in various tissues including neurons endothelial cells and the cardiovascular system. It is an integral part of the semaphorin signaling pathway interacting primarily with class 3 semaphorins and influencing cytoskeletal dynamics.

Biological function summary

Plexin D1 regulates cell movement and positioning by modulating cell adhesion and cytoskeletal rearrangement. As part of semaphorin signaling plexin D1 coordinates with other membrane proteins and signaling molecules to control processes like axon guidance and vascular patterning. It does not act alone but rather within a complex network of associated proteins that enhance its functional outcomes. Its involvement is important at the developmental stages especially within the nervous and vascular systems.

Pathways

The semaphorin-plexin signaling axis is where plexin D1 finds its significant role. It impacts the MAPK and PI3K pathways which are essential in controlling cellular responses to environmental cues. In these pathways it interacts with proteins such as Ras and Rho GTPases contributing to the regulation of cellular dynamics and survival. The interplay of plexin D1 with semaphorin 3E is particularly important for guiding cells in response to repulsive cues within the cellular environment.

Plexin D1 is associated with cancer and vascular disorders. Its role in cellular migration and angiogenesis makes it an important player in tumor progression and metastasis. Abnormal plexin D1 signaling might lead to improper blood vessel formation contributing to diseases like heart defects. In cancer its interaction with proteins like VEGF can promote tumor-induced angiogenesis exacerbating the disease state. Understanding these interactions is fundamental for developing therapeutic interventions targeting plexin D1-mediated pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E.

See full target information PLXND1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation : PubMed40533501

2025

Endothelial cells-derived SEMA3G suppresses glioblastoma stem cells by inducing c-Myc degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Xiang Min,Li-Li Feng,Yi-Xuan Zhang,Chen-Chen Jiang,Hong-Zhen Zhang,Yan Chen,Kohji Fukunaga,Fang Liu,Yu-Jie Zhang,Takuya Sasaki,Xu Qian,Katsuhisa Horimoto,Jian-Dong Jiang,Ying-Mei Lu,Feng Han

Science advances 10:eadp0684 PubMed39413197

2024

Semaphorin 3D promotes pancreatic ductal adenocarcinoma progression and metastasis through macrophage reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Noelle R J Thielman,Vanessa Funes,Sanjana Davuluri,Hector E Ibanez,Wei-Chih Sun,Juan Fu,Keyu Li,Stephen Muth,Xingyi Pan,Kenji Fujiwara, Dwayne L Thomas Ii,MacKenzie Henderson,Selina Shiqing Teh,Qingfeng Zhu,Elizabeth Thompson,Elizabeth M Jaffee,Alex Kolodkin,Fengxi Meng,Lei Zheng

Cell death & disease 14:41 PubMed36658114

2023

Plexin-A2 enables the proliferation and the development of tumors from glioblastoma derived cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shira Toledano,Adi D Sabag,Neta Ilan,Tanya Liburkin-Dan,Ofra Kessler,Gera Neufeld

Neuron 111:190-201.e8 PubMed36384142

2022

Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development.

Applications

Unspecified application

Species

Unspecified reactive species

Yixun Su,Xiaorui Wang,Yujian Yang,Liang Chen,Wenlong Xia,Kimberly K Hoi,Hui Li,Qi Wang,Guangdan Yu,Xiaoying Chen,Shouyu Wang,Yuxin Wang,Lan Xiao,Alexei Verkhratsky,Stephen P J Fancy,Chenju Yi,Jianqin Niu

Frontiers in physiology 13:960480 PubMed36017337

2022

PLXND1-mediated calcium dyshomeostasis impairs endocardial endothelial autophagy in atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjia Sun,Zhen Chen,Yuanbin Song,Bo Zhang,Jie Yang,Hu Tan

Scientific reports 11:10287 PubMed33986363

2021

Profilin2 regulates actin rod assembly in neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Marie Walter,Sebastian Rademacher,Andreas Pich,Peter Claus

Frontiers in oncology 10:604131 PubMed33489909

2021

Prognostic Value of PLXND1 and TGF-β1 Coexpression and Its Correlation With Immune Infiltrates in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juanni Li,Kuan Hu,Dongren He,Lei Zhou,Zhiming Wang,Yiming Tao

Scientific reports 9:3858 PubMed30846754

2019

Peptide vaccine for semaphorin3E ameliorates systemic glucose intolerance in mice with dietary obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Yohko Yoshida,Ippei Shimizu,Yuka Hayashi,Ryutaro Ikegami,Masayoshi Suda,Goro Katsuumi,Takayuki Wakasugi,Masaaki Nakao,Hironori Nakagami,Ryuichi Morishita,Tohru Minamino

FASEB journal : official publication of the Federa 33:4947-4961 PubMed30653356

2019

Sema3E/PlexinD1 signaling inhibits postischemic angiogenesis by regulating endothelial DLL4 and filopodia formation in a rat model of ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Fan Zhou,An-Qi Chen,Jie-Hong Wu,Ling Mao,Yuan-Peng Xia,Hui-Juan Jin,Quan-Wei He,Qing Robert Miao,Zhen-Yu Yue,Xiu-Li Liu,Ming Huang,Ya-Nan Li,Bo Hu

Neurobiology of aging 70:102-116 PubMed30007159

2018

Sema3E/PlexinD1 inhibition is a therapeutic strategy for improving cerebral perfusion and restoring functional loss after stroke in aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Fan Zhou,Peng-Cheng Li,Jie-Hong Wu,James Andrew Haslam,Ling Mao,Yuan-Peng Xia,Quan-Wei He,Xu-Xia Wang,Hao Lei,Xiao-Li Lan,Qing Robert Miao,Zhen-Yu Yue,Ya-Nan Li,Bo Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plexin D1 antibody

Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (AB28762)

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB28762)

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB28762)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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