Goat Polyclonal Plexin D1 antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PLXND1 aa 1650-1700.
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Publications
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- Neuron 111:190-201.e82022Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixun Su,Xiaorui Wang,Yujian Yang,Liang Chen,Wenlong Xia,Kimberly K Hoi,Hui Li,Qi Wang,Guangdan Yu,Xiaoying Chen,Shouyu Wang,Yuxin Wang,Lan Xiao,Alexei Verkhratsky,Stephen P J Fancy,Chenju Yi,Jianqin NiuPubMed 36384142
- Frontiers in physiology 13:9604802022PLXND1-mediated calcium dyshomeostasis impairs endocardial endothelial autophagy in atrial fibrillation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjia Sun,Zhen Chen,Yuanbin Song,Bo Zhang,Jie Yang,Hu TanPubMed 36017337
- Scientific reports 11:102872021Profilin2 regulates actin rod assembly in neuronal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Marie Walter,Sebastian Rademacher,Andreas Pich,Peter ClausPubMed 33986363
- Frontiers in oncology 10:6041312021Prognostic Value of PLXND1 and TGF-β1 Coexpression and Its Correlation With Immune Infiltrates in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanni Li,Kuan Hu,Dongren He,Lei Zhou,Zhiming Wang,Yiming TaoPubMed 33489909
- Scientific reports 9:38582019Peptide vaccine for semaphorin3E ameliorates systemic glucose intolerance in mice with dietary obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohko Yoshida et. al.PubMed 30846754
- Neurobiology of aging 70:102-1162018Sema3E/PlexinD1 inhibition is a therapeutic strategy for improving cerebral perfusion and restoring functional loss after stroke in aged rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Fan Zhou et. al.PubMed 30007159
- Journal of cell science 131:2018Complexes of plexin-A4 and plexin-D1 convey semaphorin-3C signals to induce cytoskeletal collapse in the absence of neuropilins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatyana Smolkin et. al.PubMed 29661844
- Human molecular genetics 26:3946-39592017Metalloprotease-mediated cleavage of PlexinD1 and its sequestration to actin rods in the motoneuron disease spinal muscular atrophy (SMA).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian Rademacher,Bert M Verheijen,Niko Hensel,Miriam Peters,Gamze Bora,Gudrun Brandes,Renata Vieira de Sá,Natascha Heidrich,Silke Fischer,Hella Brinkmann,W Ludo van der Pol,Brunhilde Wirth,R Jeroen Pasterkamp,Peter ClausPubMed 29016853
- Arthritis research & therapy 17:2212015Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis.
- Investigative ophthalmology & visual science 52:1819-312011Proteomic analyses of corneal tissue subjected to alkali exposure.Applications:WBReactive species:Unspecified reactive speciesToral Parikh,Natalie Eisner,Praseeda Venugopalan,Qin Yang,Byron L Lam,Sanjoy K BhattacharyaPubMed 20861482
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PLXND1 aa 1650-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D7
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E.
Alternative names
KIAA0620, PLXND1, Plexin-D1
Recommended products
Goat Polyclonal Plexin D1 antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PLXND1 aa 1650-1700.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PLXND1 aa 1650-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4D7
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plexin D1 also known as PLXND1 is a transmembrane receptor that plays an important role in cell signaling. It belongs to the plexin family and has a molecular weight of roughly 250 kDa. The plexin D1 receptor is widely expressed in various tissues including neurons endothelial cells and the cardiovascular system. It is an integral part of the semaphorin signaling pathway interacting primarily with class 3 semaphorins and influencing cytoskeletal dynamics.
- Biological function summary
Plexin D1 regulates cell movement and positioning by modulating cell adhesion and cytoskeletal rearrangement. As part of semaphorin signaling plexin D1 coordinates with other membrane proteins and signaling molecules to control processes like axon guidance and vascular patterning. It does not act alone but rather within a complex network of associated proteins that enhance its functional outcomes. Its involvement is important at the developmental stages especially within the nervous and vascular systems.
- Pathways
The semaphorin-plexin signaling axis is where plexin D1 finds its significant role. It impacts the MAPK and PI3K pathways which are essential in controlling cellular responses to environmental cues. In these pathways it interacts with proteins such as Ras and Rho GTPases contributing to the regulation of cellular dynamics and survival. The interplay of plexin D1 with semaphorin 3E is particularly important for guiding cells in response to repulsive cues within the cellular environment.
- Associated diseases and disorders
Plexin D1 is associated with cancer and vascular disorders. Its role in cellular migration and angiogenesis makes it an important player in tumor progression and metastasis. Abnormal plexin D1 signaling might lead to improper blood vessel formation contributing to diseases like heart defects. In cancer its interaction with proteins like VEGF can promote tumor-induced angiogenesis exacerbating the disease state. Understanding these interactions is fundamental for developing therapeutic interventions targeting plexin D1-mediated pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (ab28762)
Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Incubated with ab28762 for 1 hour (10 μg/ml) followed by Alexa Fluor® 488 secondary antibody (2 μg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (ab28762)
Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (ab28762)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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