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AB96313

Anti-Plexin D1 antibody

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(3 Publications)

Rabbit Polyclonal Plexin D1 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PLXND1 aa 1600 to C-terminus.

View Alternative Names

KIAA0620, PLXND1, Plexin-D1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB96313)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (AB96313)

Immunofluorescence analysis of methanol-fixed HeLa, using ab96313 at 1/500 dilution.

Western blot - Anti-Plexin D1 antibody (AB96313)
  • WB

Supplier Data

Western blot - Anti-Plexin D1 antibody (AB96313)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution

Lane 1:

Huh-7 whole cell lysates at 30 µg

Lane 2:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 212 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PLXND1 aa 1600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4D7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin D1 also known as PLXND1 is a transmembrane receptor that plays an important role in cell signaling. It belongs to the plexin family and has a molecular weight of roughly 250 kDa. The plexin D1 receptor is widely expressed in various tissues including neurons endothelial cells and the cardiovascular system. It is an integral part of the semaphorin signaling pathway interacting primarily with class 3 semaphorins and influencing cytoskeletal dynamics.
Biological function summary

Plexin D1 regulates cell movement and positioning by modulating cell adhesion and cytoskeletal rearrangement. As part of semaphorin signaling plexin D1 coordinates with other membrane proteins and signaling molecules to control processes like axon guidance and vascular patterning. It does not act alone but rather within a complex network of associated proteins that enhance its functional outcomes. Its involvement is important at the developmental stages especially within the nervous and vascular systems.

Pathways

The semaphorin-plexin signaling axis is where plexin D1 finds its significant role. It impacts the MAPK and PI3K pathways which are essential in controlling cellular responses to environmental cues. In these pathways it interacts with proteins such as Ras and Rho GTPases contributing to the regulation of cellular dynamics and survival. The interplay of plexin D1 with semaphorin 3E is particularly important for guiding cells in response to repulsive cues within the cellular environment.

Plexin D1 is associated with cancer and vascular disorders. Its role in cellular migration and angiogenesis makes it an important player in tumor progression and metastasis. Abnormal plexin D1 signaling might lead to improper blood vessel formation contributing to diseases like heart defects. In cancer its interaction with proteins like VEGF can promote tumor-induced angiogenesis exacerbating the disease state. Understanding these interactions is fundamental for developing therapeutic interventions targeting plexin D1-mediated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E.
See full target information PLXND1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 38:110483 PubMed35294878

2022

Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Szczurkowska,Alan Guo,Jacqueline Martin,Seong-Il Lee,Edward Martinez,Chia Te Chien,Tamor A Khan,Ravnit Singh,Doreen Dadson,Tracy S Tran,Sophie Pautot,Maya Shelly

Human molecular genetics 26:3946-3959 PubMed29016853

2017

Metalloprotease-mediated cleavage of PlexinD1 and its sequestration to actin rods in the motoneuron disease spinal muscular atrophy (SMA).

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Rademacher,Bert M Verheijen,Niko Hensel,Miriam Peters,Gamze Bora,Gudrun Brandes,Renata Vieira de Sá,Natascha Heidrich,Silke Fischer,Hella Brinkmann,W Ludo van der Pol,Brunhilde Wirth,R Jeroen Pasterkamp,Peter Claus

eLife 6: PubMed28537552

2017

Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex.

Applications

Unspecified application

Species

Unspecified reactive species

Guijun Shang,Chad A Brautigam,Rui Chen,Defen Lu,Jesús Torres-Vázquez,Xuewu Zhang
View all publications
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Product promise

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