Anti-PLGF antibody [EPR29637-148]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLGF antibody [EPR29637-148] (AB322267)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a PGF expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling PLGF with ab322267 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a PGF expression vector containing a Myc-His tag, negative staining on (B) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab322267 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLGF antibody [EPR29637-148] (AB322267)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PLGF with ab322267 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse lung. The section was incubated with ab322267 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLGF antibody [EPR29637-148] (AB322267)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PLGF with ab322267 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cerebrum. The section was incubated with ab322267 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLGF antibody [EPR29637-148] (AB322267)

Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling PLGF with ab322267 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse placenta. The section was incubated with ab322267 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLGF antibody [EPR29637-148] (AB322267)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling PLGF with ab322267 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse colon. The section was incubated with ab322267 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-PLGF antibody [EPR29637-148] (AB322267)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse placenta tissue staining PLGF with ab322267 at a 1/2000 dilution, ab324045 anti-AP2 gamma/TFAP2C used at 1/500 dilution and ab182981 anti-CD31 used at a 1/5000 dilution.

Panel A : merged staining of anti-PLGF (green; Opal™520), anti-AP2 gamma/TFAP2C (magenta; Opal™690) and anti-CD31 (gray; Opal™570) on mouse placenta.

Panel B : anti-PLGF showed positive staining in mouse placenta.

Panel C : anti-AP2 gamma/TFAP2C staining trophoblast stem cells in mouse placenta.

Panel D : anti-CD31 staining endothelium in mouse placenta.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322267, ab324045 and ab182981 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Dot

Supplier Data

Dot Blot - Anti-PLGF antibody [EPR29637-148] (AB322267)

Dot blot analysis of PLGF using ab322267 at 1 : 1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged mouse PGF fragment
Lane2 : His-tagged mouse VEFG fragment

Exposure time : 48 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse VEGF by dot blot.

#In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-PLGF antibody [EPR29637-148] (ab322267) at 1/1000 dilution

Lane 1:

His-tagged mouse PGF fragment

Lane 2:

His-tagged mouse VEFG fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 48s