AB17057

Anti-PLK1 antibody [36-298]

Name: Anti-PLK1 antibody [36-298] Mouse monoclonal (ab17057) | Abcam
Brand: Abcam Limited
SKU: AB17057
Rating: 4 (3 reviews)

(3 Reviews)

(44 Publications)

Mouse Monoclonal PLK1 antibody. Suitable for ICC, Flow Cyt (Intra), WB and reacts with Mouse, Human, Recombinant full length protein samples. Cited in 44 publications.

View Alternative Names

PLK, PLK1, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, Serine/threonine-protein kinase 13, PLK-1, STPK13

8 Images

Collaborator

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

In panel one HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were stained with ab17057 (green) and DAPI. In the second panel, cells were stained with ab17057 (green) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeabilized for 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4°C diluted 1/400 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen.

Notes : Ample washing between each step.

TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl₂.

This image is courtesy of Scott Slattery and Mike Mancini

Unknown

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

Immunofluoresence using ab17057 and either HeLa S3, NIH/3T3 (Mouse embyro fibroblast cell line) or U-2 OS (Human bone osteosarcoma epithelial cell line) cells.

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)

Flow cytometry overlay histogram showing HeLa cells stained with ab17057 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab17057) (1x10⁶ in 100 μl at 5 μg/ml) for 30 min at 22°C.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was mouse IgG1 kappa; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Lab

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

ab17057 staining PLK1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17057 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was mouse IgG1 kappa; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Project

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

All lanes:

Western blot - Anti-PLK1 antibody [36-298] (ab17057) at 1 µg/mL

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 66 kDa

true

Exposure time: 2min

Unknown

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-PLK1 antibody [36-298] (ab17057)

Lane 1:

Recombinant PLK1

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) cell extract

Lane 3:

HeLa S3 cell extract

Predicted band size: 68 kDa

Observed band size: 66 kDa

false

CiteAb

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

PLK1 western blot using anti-PLK1 antibody [36-298] ab17057. Publication image and figure legend from Tan, E. P., Caro, S., et al., 2013, J Biol Chem, PubMed 23946484.

ab17057 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab17057 please see the product overview.

Disrupted O-GlcNAc cycling alters spindle protein expression. a spindle schematic of the locations and area of phosphorylation of mitotic kinases AurA, AurB, and PLK1. b, M phase synchronized GFP/OGT/OGA gain of function or TMG-treated cells were Western blotted for PLK1, AurA, and actin. c, DNA (blue), α-tubulin (green), and AurA (red) were confocal imaged at M phase in OGT/OGA gain of function cells. d, DNA (blue), α-tubulin (green), and PLK1 (red) were confocal imaged at M phase in OGT/OGA gain of function cells. e, Western blot of CPC proteins AurB, INCENP, borealin, surviving, and actin as a load control (f) DNA (blue), INCENP (green), and AurB (red) were confocal imaged at M phase in OGT/OGA gain of function cells. g, AurB width was quantified using ImageJ (mean ± S.E., replicate number (n) : nControl = 45, nOGT = 35, nOGA = 43, *, p < 0.005 between Control versus OGA). h, DNA (blue), INCENP (green), and survivin (red) were confocal imaged at M phase in OGT/OGA gain of function cells.

false

Carrier free

Anti-PLK1 antibody [36-298] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

36-298

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

aa330-370.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "5 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

style

left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 23455478, PubMed : 23509069, PubMed : 28512243, PubMed : 8991084). Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 23455478, PubMed : 23509069, PubMed : 28512243, PubMed : 8991084). Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, MRE11, PPP1R12A/MYPT1, POLQ, PRC1, RACGAP1/CYK4, RAD51, RHNO1, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17218258, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 22325354, PubMed : 23455478, PubMed : 23509069, PubMed : 25986610, PubMed : 26811421, PubMed : 28512243, PubMed : 37440612, PubMed : 37674080, PubMed : 8991084). Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL (PubMed : 16980960, PubMed : 19509060). NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (PubMed : 19509060). Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins (PubMed : 12852856). Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1 (PubMed : 12939256, PubMed : 16247472, PubMed : 17351640, PubMed : 19468300, PubMed : 19468302). Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains (PubMed : 12939256, PubMed : 17351640). Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation (PubMed : 19468300, PubMed : 19468302). Promotes the central spindle recruitment of ECT2 (PubMed : 16247472). Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1 (PubMed : 11202906, PubMed : 12447691, PubMed : 12524548, PubMed : 19160488). Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1 (PubMed : 11202906). Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase (PubMed : 12447691, PubMed : 12524548). Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity (PubMed : 19160488). Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2 (PubMed : 15148369, PubMed : 15469984, PubMed : 17376779, PubMed : 18331714). PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation (PubMed : 17617734). Required for kinetochore localization of BUB1B (PubMed : 17376779). Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2 (By similarity). Phosphorylates SGO1 : required for spindle pole localization of isoform 3 of SGO1 and plays a role in regulating its centriole cohesion function (PubMed : 18331714). Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome (PubMed : 15148369, PubMed : 15469984). Acts as a negative regulator of p53 family members : phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53 (PubMed : 19473992). Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA (PubMed : 18521620). Contributes to the regulation of AURKA function (PubMed : 18615013, PubMed : 18662541). Also required for recovery after DNA damage checkpoint and entry into mitosis (PubMed : 18615013, PubMed : 18662541). Phosphorylates MISP, leading to stabilization of cortical and astral microtubule attachments required for proper spindle positioning (PubMed : 23509069). Together with MEIKIN, acts as a regulator of kinetochore function during meiosis I : required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage (By similarity). Phosphorylates CEP68 and is required for its degradation (PubMed : 25503564). Regulates nuclear envelope breakdown during prophase by phosphorylating DCTN1 resulting in its localization in the nuclear envelope (PubMed : 20679239). Phosphorylates the heat shock transcription factor HSF1, promoting HSF1 nuclear translocation upon heat shock (PubMed : 15661742). Phosphorylates HSF1 also in the early mitotic period; this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex induicing HSF1 degradation, and hence mitotic progression (PubMed : 18794143). Regulates mitotic progression by phosphorylating RIOK2 (PubMed : 21880710). Through the phosphorylation of DZIP1 regulates the localization during mitosis of the BBSome, a ciliary protein complex involved in cilium biogenesis (PubMed : 27979967). Regulates DNA repair during mitosis by mediating phosphorylation of POLQ and RHNO1, thereby promoting POLQ recruitment to DNA damage sites (PubMed : 37440612, PubMed : 37674080). Phosphorylates ATXN10 which may play a role in the regulation of cytokinesis and may stimulate the proteasome-mediated degradation of ATXN10 (PubMed : 21857149).

See full target information PLK1

style

left-column

Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7898 PubMed40849432

2025

The cAMP-PKA signaling initiates mitosis by phosphorylating Bora.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhu,Shinan Zhou,Yingqi Zhang,Qinfu Chen,Xueying Yuan,Long Zhang,Haiyan Yan,Fangwei Wang

General physiology and biophysics 44:259-274 PubMed40567070

2025

Knockdown of PLK1 suppresses malignant phenotypes and tumor growth in bladder cancer via activating Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yang,Yunhan Wang,Zhou Zheng,Hanchao Zhang,Haifeng Hu

Journal of inflammation research 18:4381-4394 PubMed40162075

2025

PLK1 Mediates the Proliferation and Contraction of Airway Smooth Muscle Cells and Has a Role in T2-High Asthma with Neutrophilic Inflammation Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Pan,Yishu Xue,Xia Fei,Lei Zhao,Lei Han,Hang Su,Yanmei Lin,Yan Zhou,Yingying Zhang,Guogang Xie,Deping Kong,Wuping Bao,Min Zhang

Scientific reports 15:9280 PubMed40102598

2025

Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Dohyong Lee,Sungjin Ryu,Ji Hwa Hea,Globinna Kim,In-Jeoung Baek,Young Hoon Sung,Kunsoo Rhee

Life science alliance 8: PubMed39433344

2024

CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Weixia Kong,Masatoshi Hara,Yurika Tokunaga,Kazuhiro Okumura,Yasuhiro Hirano,Jiahang Miao,Yusuke Takenoshita,Masakazu Hashimoto,Hiroshi Sasaki,Toshihiko Fujimori,Yuichi Wakabayashi,Tatsuo Fukagawa

The EMBO journal 43:2424-2452 PubMed38714893

2024

A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yan,Xueying Yuan,Mingjie Liu,Qinfu Chen,Miao Zhang,Junfen Xu,Ling-Hui Zeng,Long Zhang,Jun Huang,Weiguo Lu,Xiaojing He,Haiyan Yan,Fangwei Wang

Cell proliferation 57:e13590 PubMed38084791

2023

METTL16-mediated N6-methyladenosine modification of Soga1 enables proper chromosome segregation and chromosomal stability in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Li,Fang Yang,Zeyu Wang,Siqing Zheng,Shuang Zhang,Chen Wang,Bing He,Jia-Bei Wang,Hao Wang

The EMBO journal 42:e112630 PubMed37712330

2023

A bifunctional kinase-phosphatase module balances mitotic checkpoint strength and kinetochore-microtubule attachment stability.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Corno,Marilia H Cordeiro,Lindsey A Allan,Qian-Wei Lim,Elena Harrington,Richard J Smith,Adrian T Saurin

Cell death & disease 14:571 PubMed37640723

2023

Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Du,Yaqiong Shang,Yun Qian,Yan Guo,Shuang Chen,Xiuli Lin,Weidong Cao,Xiaomei Tang,Anning Zhou,Songming Huang,Aihua Zhang,Zhanjun Jia,Yue Zhang

Frontiers in oncology 12:985827 PubMed36818671

2023

Identification of NLE1/CDK1 axis as key regulator in the development and progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Xu,Lei Wang,Bin Mo,Xiao Xie,Rui Hu,Lianyong Jiang,Fengqing Hu,Fangbao Ding,Haibo Xiao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLK1 antibody [36-298]

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)

Immunocytochemistry - Anti-PLK1 antibody [36-298] (AB17057)

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] (AB17057)

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

Western blot - Anti-PLK1 antibody [36-298] (AB17057)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (44)

Product promise