AB178666

Anti-PLK1 antibody [36-298] - BSA and Azide free

Name: Anti-PLK1 antibody [36-298] - BSA and Azide free (ab178666) | Abcam
Brand: Abcam Limited
SKU: AB178666

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(6 Publications)

Mouse Monoclonal PLK1 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra), WB and reacts with Human, Mouse, Recombinant full length protein samples. Cited in 6 publications.

View Alternative Names

PLK, PLK1, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, Serine/threonine-protein kinase 13, PLK-1, STPK13

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Lab

Immunocytochemistry - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab17057).

ab17057 staining PLK1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17057 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

Flow cytometry overlay histogram showing HeLa cells stained with ab17057 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab17057) (1x106 in 100 μl at 5 μg/ml) for 30 min at 22°C.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was mouse IgG1 kappa; (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

CiteAb

Western blot - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

Western Blotting using Anti-PLK1 antibody [36-298] - BSA and Azide free, ab178666. Publication image from Liccardi, G. et al., 2019, Mol Cell, 30598363. Legend direct from paper.

RIPK1 Negatively Regulates PLK1-Mediated Phosphorylation of BUBR1(A) Scheme illustrating how mitotic ripoptosome interacts and modulates PLK1 and how pharmacological inhibition regulates such interaction and downstream substrate activation. Immunofluorescence analysis using anti-BUBR1 or anti-BUBR1-pT680 antibodies. HT1080 cells were synchronized with CDK1i and released into media containing the indicated agents. Scale bars : 10 µm.(B) HT1080 cells were synchronized with CDK1i and released. Lysates from asynchronous or synchronized HT1080 cells were immunoprecipitated with anti-PLK1 antibody. Immunoblot analysis using the indicated antibodies is shown.(C and D) In situ PLA detection of PLK1/BUBR1 (C) or PLK1/BUBR1-pT680 (D) in synchronized HT1080 cells, treated with the indicated agents. Scale bars : 10 µm.(E) Immunofluorescence analysis using anti-BUBR1-pT680 antibodies (under extraction conditions) in CDK1i-synchronized HT1080 (left) and HT29 (right) cells under the indicated RNAi conditions. Cells were released for 30 min, after which MG132 was added for 90 min to arrest cells in metaphase. N.T. indicates non-targeting RNAi Control oligos. Scale bars : 10 µm.(F) Western blot analysis of phosphorylated BUBR1 following knockdown of Control (Ctrl), Ripk1, or Plk1 in CDK1i-synchronized and released HT1080 cells.(G) CDK1i-synchronized HT1080 cells were released into media containing the indicated agents. Cells were released for 30 min, after which MG132 was added for 90 min to arrest cells in metaphase. Only cells presenting mitotic abnormalities were scored for PLK1 localization. Images show representative examples of PLK1 mis-localization. Scale bars : 10 µm.

false

Unconjugated

Anti-PLK1 antibody [36-298]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

36-298

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

aa330-370.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 23455478, PubMed : 23509069, PubMed : 28512243, PubMed : 8991084). Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 23455478, PubMed : 23509069, PubMed : 28512243, PubMed : 8991084). Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, MRE11, PPP1R12A/MYPT1, POLQ, PRC1, RACGAP1/CYK4, RAD51, RHNO1, SGO1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, WEE1 and HNRNPU (PubMed : 11202906, PubMed : 12207013, PubMed : 12447691, PubMed : 12524548, PubMed : 12738781, PubMed : 12852856, PubMed : 12939256, PubMed : 14532005, PubMed : 14734534, PubMed : 15070733, PubMed : 15148369, PubMed : 15469984, PubMed : 16198290, PubMed : 16247472, PubMed : 16980960, PubMed : 17081991, PubMed : 17218258, PubMed : 17351640, PubMed : 17376779, PubMed : 17617734, PubMed : 18174154, PubMed : 18331714, PubMed : 18418051, PubMed : 18477460, PubMed : 18521620, PubMed : 18615013, PubMed : 19160488, PubMed : 19351716, PubMed : 19468300, PubMed : 19468302, PubMed : 19473992, PubMed : 19509060, PubMed : 19597481, PubMed : 22325354, PubMed : 23455478, PubMed : 23509069, PubMed : 25986610, PubMed : 26811421, PubMed : 28512243, PubMed : 37440612, PubMed : 37674080, PubMed : 8991084). Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL (PubMed : 16980960, PubMed : 19509060). NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (PubMed : 19509060). Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins (PubMed : 12852856). Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, CENPU, PRC1 and RACGAP1 (PubMed : 12939256, PubMed : 16247472, PubMed : 17351640, PubMed : 19468300, PubMed : 19468302). Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains (PubMed : 12939256, PubMed : 17351640). Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation (PubMed : 19468300, PubMed : 19468302). Promotes the central spindle recruitment of ECT2 (PubMed : 16247472). Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1 (PubMed : 11202906, PubMed : 12447691, PubMed : 12524548, PubMed : 19160488). Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1 (PubMed : 11202906). Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase (PubMed : 12447691, PubMed : 12524548). Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity (PubMed : 19160488). Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2 (PubMed : 15148369, PubMed : 15469984, PubMed : 17376779, PubMed : 18331714). PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation (PubMed : 17617734). Required for kinetochore localization of BUB1B (PubMed : 17376779). Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2 (By similarity). Phosphorylates SGO1 : required for spindle pole localization of isoform 3 of SGO1 and plays a role in regulating its centriole cohesion function (PubMed : 18331714). Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome (PubMed : 15148369, PubMed : 15469984). Acts as a negative regulator of p53 family members : phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53 (PubMed : 19473992). Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA (PubMed : 18521620). Contributes to the regulation of AURKA function (PubMed : 18615013, PubMed : 18662541). Also required for recovery after DNA damage checkpoint and entry into mitosis (PubMed : 18615013, PubMed : 18662541). Phosphorylates MISP, leading to stabilization of cortical and astral microtubule attachments required for proper spindle positioning (PubMed : 23509069). Together with MEIKIN, acts as a regulator of kinetochore function during meiosis I : required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage (By similarity). Phosphorylates CEP68 and is required for its degradation (PubMed : 25503564). Regulates nuclear envelope breakdown during prophase by phosphorylating DCTN1 resulting in its localization in the nuclear envelope (PubMed : 20679239). Phosphorylates the heat shock transcription factor HSF1, promoting HSF1 nuclear translocation upon heat shock (PubMed : 15661742). Phosphorylates HSF1 also in the early mitotic period; this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex induicing HSF1 degradation, and hence mitotic progression (PubMed : 18794143). Regulates mitotic progression by phosphorylating RIOK2 (PubMed : 21880710). Through the phosphorylation of DZIP1 regulates the localization during mitosis of the BBSome, a ciliary protein complex involved in cilium biogenesis (PubMed : 27979967). Regulates DNA repair during mitosis by mediating phosphorylation of POLQ and RHNO1, thereby promoting POLQ recruitment to DNA damage sites (PubMed : 37440612, PubMed : 37674080). Phosphorylates ATXN10 which may play a role in the regulation of cytokinesis and may stimulate the proteasome-mediated degradation of ATXN10 (PubMed : 21857149).

See full target information PLK1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 73:413-428.e7 PubMed30598363

2019

RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Gianmaria Liccardi,Laura Ramos Garcia,Tencho Tenev,Alessandro Annibaldi,Arnaud J Legrand,David Robertson,Rebecca Feltham,Holly Anderton,Maurice Darding,Nieves Peltzer,Marius Dannappel,Hannah Schünke,Luca L Fava,Manuel D Haschka,Timo Glatter,Alexey Nesvizhskii,Alexander Schmidt,Philip A Harris,John Bertin,Peter J Gough,Andreas Villunger,John Silke,Manolis Pasparakis,Katiuscia Bianchi,Pascal Meier

BMC cancer 11:71 PubMed21324136

2011

Aberrant methylation of Polo-like kinase CpG islands in Plk4 heterozygous mice.

Applications

Species

Mouse

Alejandra Ward,Alan Morettin,David Shum,John W Hudson

The Journal of biological chemistry 285:29556-68 PubMed20615875

2010

Plk1 regulates both ASAP localization and its role in spindle pole integrity.

Applications

WB, ICC/IF

Species

Human, Human

Grégory Eot-Houllier,Magali Venoux,Sophie Vidal-Eychenié,Minh-Thâo Hoang,Dominique Giorgi,Sylvie Rouquier

Cancer research 70:3657-66 PubMed20406977

2010

Inactivation of DNA-dependent protein kinase leads to spindle disruption and mitotic catastrophe with attenuated checkpoint protein 2 Phosphorylation in response to DNA damage.

Applications

Species

Human

Zeng-Fu Shang,Bo Huang,Qin-Zhi Xu,Shi-Meng Zhang,Rong Fan,Xiao-Dan Liu,Yu Wang,Ping-Kun Zhou

The Journal of biological chemistry 284:2344-53 PubMed19033445

2008

Deficiency in chromosome congression by the inhibition of Plk1 polo box domain-dependent recognition.

Applications

ICC/IF

Species

Human

Nobumoto Watanabe,Tomomi Sekine,Masatoshi Takagi,Jun-ichi Iwasaki,Naoko Imamoto,Hisashi Kawasaki,Hiroyuki Osada

The Journal of cell biology 171:431-6 PubMed16260496

2005

Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoya Yamaguchi,Hidemasa Goto,Tomoya Yokoyama,Herman Silljé,Anja Hanisch,Andreas Uldschmid,Yasushi Takai,Takashi Oguri,Erich A Nigg,Masaki Inagaki

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Anti-PLK1 antibody [36-298] - BSA and Azide free

Immunocytochemistry - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

Flow Cytometry (Intracellular) - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

Western blot - Anti-PLK1 antibody [36-298] - BSA and Azide free (AB178666)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise