Rabbit Polyclonal PLK4 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLK4 aa 600-900.
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Publications
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- The Prostate 82:957-9692022PLK4 is upregulated in prostate cancer and its inhibition reduces centrosome amplification and causes senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChandra K Singh,Ryan A Denu,Minakshi Nihal,Maria Shabbir,Debra R Garvey,Wei Huang,Kenneth A Iczkowski,Nihal AhmadPubMed 35333404
- Scientific reports 10:119212020Analysis of the "centrosome-ome" identifies MCPH1 deletion as a cause of centrosome amplification in human cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan A Denu,Mark E BurkardPubMed 32681070
- Journal of cellular and molecular medicine 24:3931-39472020Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Dengpeng Ren,Yan Sun,Chao Xu,Chunhong Wang,Rui Cheng,Lina Wang,Guijun Jia,Jinrui Ren,Jiuhong Ma,Yue Tu,Hongming JiPubMed 32126150
- Cancer medicine 8:6476-64842019A cis-eQTL genetic variant in PLK4 confers high risk of hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Meng et. al.PubMed 31489978
- Cell death & disease 10:5702019Cep131 overexpression promotes centrosome amplification and colon cancer progression by regulating Plk4 stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Hyun Kim et. al.PubMed 31358734
- Proceedings of the National Academy of Sciences of 116:11309-113182019Role for polo-like kinase 4 in mediation of cytokinesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael F Press et. al.PubMed 31097597
- Oncology reports 41:2159-21672019Polo‑like kinase 4 promotes tumorigenesis and induces resistance to radiotherapy in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Wang et. al.PubMed 30816483
- Molecular medicine reports 18:5327-53362018High LIN28A and PLK4 co‑expression is associated with poor prognosis in epithelial ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao He et. al.PubMed 30365085
- Molecular cancer research : MCR 16:517-5272018Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan A Denu et. al.PubMed 29330283
- European journal of human genetics : EJHG 25:1118-11252017Analysis of centrosome and DNA damage response in PLK4 associated Seckel syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTuba Dinçer et. al.PubMed 28832566
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PLK4 aa 600-900. The exact immunogen used to generate this antibody is proprietary information. Database link O00444
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124). Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites (PubMed:30804208).
Alternative names
SAK, STK18, PLK4, Serine/threonine-protein kinase PLK4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak, PLK-4
Recommended products
Rabbit Polyclonal PLK4 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLK4 aa 600-900.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PLK4 aa 600-900. The exact immunogen used to generate this antibody is proprietary information. Database link O00444
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody (ab137398)
Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab137398 at 1/500 dilution (Green). Alpha-tubulin filaments labeled (Red).
Immunocytochemistry - Anti-PLK4 antibody (ab137398)
Immunocytochemical analysis of methanol-fixed 233T cells labelled with ab137398 at 1/500 dilution.
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