Rabbit Recombinant Monoclonal PLK4 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (AB315223), expandable thumbnail](https://content.abcam.com/products/images/plk4-antibody-epr27156-51-bsa-and-azide-free-ab315223--western-blot-img424195.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (AB315223)")
![Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (AB315223), expandable thumbnail](https://content.abcam.com/products/images/plk4-antibody-epr27156-51-bsa-and-azide-free-ab315223--western-blot-img424194.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (AB315223)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124). Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites (PubMed:30804208).
Alternative names
SAK, STK18, PLK4, Serine/threonine-protein kinase PLK4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak, PLK-4
Recommended products
Rabbit Recombinant Monoclonal PLK4 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27156-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315223 is the carrier-free version of Anti-PLK4 antibody [EPR27156-51] ab315222.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223), expandable thumbnail](https://content.abcam.com/products/images/plk4-antibody-epr27156-51-bsa-and-azide-free-ab315223--western-blot-img424195.jpg "Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223)")
Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223)
This data was developed using Anti-PLK4 antibody [EPR27156-51] ab315222, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Loss of PLK4 autophosphorylation by CFI-400945 prevents the autoregulated destruction of PLK4, resulting in an increase in protein levels at the centriole (PMID: 25043604).
The identity of the bands between 25 kDa and 75 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PLK4 antibody [EPR27156-51] (Anti-PLK4 antibody [EPR27156-51] ab315222) at 1/1000 dilution
Lane 1: Untreated MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 50 µg
Lane 2: MOLT-4 treated with 500nM CFI-400945 for 18h whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 105 kDa, 36 kDa
Exposure time: 156s
![Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223), expandable thumbnail](https://content.abcam.com/products/images/plk4-antibody-epr27156-51-bsa-and-azide-free-ab315223--western-blot-img424194.jpg "Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223)")
Western blot - Anti-PLK4 antibody [EPR27156-51] - BSA and Azide free (ab315223)
This data was developed using Anti-PLK4 antibody [EPR27156-51] ab315222, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Loss of PLK4 autophosphorylation by CFI-400945 prevents the autoregulated destruction of PLK4, resulting in an increase in protein levels at the centriole (PMID: 25043604).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PLK4 antibody [EPR27156-51] (Anti-PLK4 antibody [EPR27156-51] ab315222) at 1/1000 dilution
Lane 1: Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 2: U-2 OS treated with 500nM CFI-400945 for 24h whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 105 kDa, 36 kDa
Exposure time: 180s
Downloads
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