AB180131

Anti-PLP2 antibody [EPR14238(B)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal PLP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

A4, PLP2, Proteolipid protein 2, Differentiation-dependent protein A4, Intestinal membrane A4 protein

4 Images

Flow Cytometry (Intracellular) - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Intracellular Flow Cytometry analysis ofU87-MG cells labeling PLP2 using ab180131 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit IgG.

Immunocytochemistry/ Immunofluorescence - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Immunofluorescence analysis of SW480 cells labeling PLP2 using ab180131 at 1/250 dilution. A Goat anti rabbit IgG (Dylight 488) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain : DAPI.

Supplier Data

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Immunoprecipitation analysis of Human colon cancer lysate labeling PLP2 using ab180131 at 1/30 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] (ab180131)

Predicted band size: 13 kDa,134 kDa,17 kDa

false

Supplier Data

Western blot - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

All lanes:

Western blot - Anti-PLP2 antibody [EPR14238(B)] (ab180131) at 1/5000 dilution

Lane 1:

Human colon cancer tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 17 kDa

false

Carrier free

Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free
660 APC

APC Anti-PLP2 antibody [EPR14238(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PLP2 antibody [EPR14238(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PLP2 antibody [EPR14238(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PLP2 antibody [EPR14238(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PLP2 antibody [EPR14238(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PLP2 antibody [EPR14238(B)]
578 PE

PE Anti-PLP2 antibody [EPR14238(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14238(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell differentiation in the intestinal epithelium.

See full target information PLP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:5330-5345 PubMed33859750

2021

CD45ROCD8 T cell-derived exosomes restrict estrogen-driven endometrial cancer development via the ERβ/miR-765/PLP2/Notch axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jie Zhou,Jie Zhang,Feng Xie,Jiang-Nan Wu,Jiang-Feng Ye,Jian Wang,Ke Wu,Ming-Qing Li

International journal of molecular sciences 19: PubMed30373180

2018

Proteolipid Protein 2 Overexpression Indicates Aggressive Tumor Behavior and Adverse Prognosis in Human Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Hsuan Chen,Dueng-Yuan Hueng,Wen-Chiuan Tsai

Medicine 94:e1327 PubMed26287415

2015

Loss of MiR-664 Expression Enhances Cutaneous Malignant Melanoma Proliferation by Upregulating PLP2.

Applications

Species

Unspecified reactive species

Zhenhua Ding,Sun Jian,Xuebiao Peng,Yimin Liu,Jianyu Wang,Li Zheng,Chengshan Ou,Yinghui Wang,Weixia Zeng,Meijuan Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLP2 antibody [EPR14238(B)]

Flow Cytometry (Intracellular) - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Immunocytochemistry/ Immunofluorescence - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Western blot - Anti-PLP2 antibody [EPR14238(B)] (AB180131)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise