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AB250143

Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PLP2 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

A4, PLP2, Proteolipid protein 2, Differentiation-dependent protein A4, Intestinal membrane A4 protein

4 Images
Flow Cytometry (Intracellular) - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)

This data was developed using ab180131, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of U87-MG cells labeling PLP2 using ab180131 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit IgG.

Immunocytochemistry/ Immunofluorescence - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)

This data was developed using ab180131, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of SW480 cells labeling PLP2 using ab180131 at 1/250 dilution. A Goat anti rabbit IgG (Dylight 488) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain : DAPI.

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)
  • IP

Supplier Data

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)

This data was developed using ab180131, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Human colon cancer lysate labeling PLP2 using ab180131 at 1/30 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-PLP2 antibody [EPR14238(B)] (<a href='/en-us/products/primary-antibodies/plp2-antibody-epr14238b-ab180131'>ab180131</a>)

Predicted band size: 13 kDa,134 kDa,17 kDa

false

Western blot - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)
  • WB

Supplier Data

Western blot - Anti-PLP2 antibody [EPR14238(B)] - BSA and Azide free (AB250143)

This data was developed using ab180131, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PLP2 antibody [EPR14238(B)] (<a href='/en-us/products/primary-antibodies/plp2-antibody-epr14238b-ab180131'>ab180131</a>) at 1/5000 dilution

Lane 1:

Human colon cancer tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14238(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250143 is the carrier-free version of ab180131.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in cell differentiation in the intestinal epithelium.
See full target information PLP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com